【Zenogen Pharma】CELLBANKER® 2

【Zenogen Pharma】CELLBANKER® 2


Description

(Serum-free cryopreservation medium)


CELLBANKER® 2 is a serum-free cell cryopreservation medium with unique complete formulation for broad spectrum of mammalian cell cultures. The unique formulation allows for stable cryopreservation and high viability after freeze-thaw procedure, without potential risk of contamination serum.

Optimum for serum-free cultured cells and peptide/protein expressing cells cryopreservation.

Package:100ml/bottle


Advantages


Safe and Save!

Safe: high cell viability, no risk of contamination serum derived pathogens, batch-to-batch stability.

Save: complete formulation, no further preparation, direct freezing at -80℃, no programmable freezer required, etc.


Protocol


Freezing

For optimum results, cells for cryopreservation should be in log phase of growth. Similar or standard freezing protocols may be substituted.

1.Examine and make sure the cell culture free of contamination, in healthy situation and proper confluency, etc.

2.Perform a cell count to determine the viability of cells.

3.Gently pellet the cells centrifugation (3 - 5 minutes at 1,000~2,000rpm, 4℃). Remove the supernatant using a aspirator.

4.Gently suspend the cells with CELLBANKER® cryopreservation medium (1 ml for 5x105 - 5x106 cells).

5.Dispense the cell suspension in 1ml aliquots to cryopreservation vials that have been labeled with the cell line name, cell concentration, passage date and other essential information.

6.Place the vials directly in a -80℃ for storage. If necessary, transfer the frozen vials to a liquid nitrogen storage tank after the vials have been frozen for at least 24 hours.


IMPORTANT: Optimum protocol may change with the cell types.



Thawing


1.Remove the frozen cell from storage and quickly thaw in a 37℃ shaking waterbath.

2.Immediately dilute and gently mix each 1ml of cells with 10ml of complete cell culture medium.

3.Gently pellet the cells centrifugation (3-5 minutes at 1,000 - 2,000rpm, 4℃). Remove the supernatant aspirator.

4.Gently suspend the cells with appropriate volume of complete cell culture medium. And plate in a culture flask.

5.Continue the further culture procedures according to standard protocols.


Storage and Stability


Store at 2 to 8 or below -20 degrees Celsius.In unopened condition and under storage temperatures (2-8℃), the product will be stable for 3 years after the date of manufacture.


Application


Cell type

Description

293

Sheared human Ad5 DNA-transformed cell line.

293T

Human cell line expressing SV40 large T antigen

32D

Murine myeloid cell

3LL

Murine Lewis lung cancer cell

A10

Thoracic aortic smooth muscle of an embryonic rat

A2781

Glioblastoma cell

Ac2F

Rat liver cell

AtT20

Murine anterior pituitary cell

Ba/F3

Murine Pro-B cell

BHK-21

Ba Hamster renal fibroblast cell

C2C12

Mouse myoblast cell

C3H10T1/2

Murine embryonic fibroblast cell

Caco-2

Human colonic adenocarcinoma cell

CHL/1U

Neonatal Chinese Hamster Lung cell

CHO

Chinese Hamster Ovary cell

CHO-K1

Chinese Hamster Ovary cell

Colo203

Human colonic adenocarcinoma cell

COS1

African green monkey kidney cell

COS7

African green monkey kidney cell

CTLL-2

Murine T-cell

DLP-1

Human colonic adenocarcinoma cell

DT40

Chicken B lymphocyte cell

DU145

Human prostate carcinoma cell

EBV transformed B cell

EJ-1

Human bladder carcinoma

ELM-D

Murine erythroblastic leukemia cell

HeLa cell

Human Uterine Cervical Carcinoma Cell

Hep3B

Human hepatoma cell

Hepal-6

Murine hepatoma cell

Hepatocyte

HepG2

Hepatocellular carcinoma cell

HL-60

Human promyelocytic leukemia cell

HT-2

Murine cell

Huh-7

Human hepatocarcinoma cell

Jurkat

Human T-cell line

K-562

Human Caucasian Chronic myelogenous leukaemia cell

Kato-III

Human stomach carcinoma

Keratinocyte

KM12-LX

Human Caucasian Chronic myelogenous leukaemia cell

L5178Y

Murine lymphoma cell

L929

Murine fibroblast cell

LM

Murine fibroblast cell

LNCap

Human prostate adenocarcinoma cell

Lymphocyte

MCF-7

Human metastatic mammary carcinoma cell

MDCK

Madin-Dar canine kidney聽 cell

Microvascular Endothelial Cell

Molt-4

Human T-cell

NB-1/GOTO

Human neuroblastoma

NCI-H441

Human lung adenocarcinoma epithelial cell

NIH3T3

Mouse embryonic fibroblast cell

P388

Murine leukimia cell

P3/x63-Ag8.U1

Murine myeloma cell

PC12

Rat pheochromocytoma cell

Periodontal ligament fibroblast cell

Periodontal ligament membrane fibroblast cell

Raji

Human B-cell line

RAW264.7

Murine Macrophage cell

sf9

Insect cell

SK-N-MC

Neuroepithelioma cell

SN12C

Renal carcinoma cell Subclone: PM6,Clone8, MM3

Stem cell

Stomach carcinoma cell

THle3

Human liver cell

T24

Human bladder carcinoma

Vero

African green monkey kidney epithelial cell

WEHI3B

Murine myeloid leukemia

WiDr

Human colon adenocarcinoma cell


Typical experimental results

Cells

Preservation period(year)

Viability of cells (%)(preservation temperature -80℃)

MOUSE

Hybridome

5

90

Myeloma

5

90

L929

5

90

FM3A

5

90

BALB/3T3

5

90

RAT

RLC-16

5

90

NKR

3

90

HAMSTER

CHO

3

90

V79

3

90

MONKEY

COS-1

3

90

Vero

5

90

HUMAN

EBV transformed cell

5

90

Fibroblast

5

90

Melanoma

5

90

SK-007

5

90

Jurkat

5

90

K562

5

90

其他细胞品类


免疫细胞系统

人肝脏细胞系统

人脐带细胞系统

人神经细胞系统

骨骼细胞系统

心脏细胞系统

内分泌细胞系统

眼睛细胞系统

皮肤细胞系统

脂肪细胞系统

脾脏细胞系统

尿道细胞系统

扁桃体细胞系统

甲状腺细胞系统

肾脏细胞系统

肺细胞系统

口腔细胞系统

胃肠道细胞系统

听觉细胞系统

毛发细胞系统

男性生殖细胞系统

女性生殖细胞系统

人巨噬细胞

人周细胞




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