【Zenogen Pharma】CELLBANKER® 1

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Zenogen Pharma Zenogen CELLBANKER® 1

Description

(Serum containing cryopreservation medium）

CELLBANKER® 1 is a ready-to-use cell cryopreservation medium with serum containing formulation for broad spectrum of mammalian cell cultures. The unique formulation allows for stable cryopreservation and high viability after freeze-thaw procedure, even for sensitive cell lines.

Package:100ml/bottle

Advantages

Safe and Save!

Safe: high cell viability
Save: complete formulation, no further preparation, direct freezing at -80℃, no programmable freezer required, etc.

Protocol

Freezing

For optimum results, cells for cryopreservation should be in log phase of growth. Similar or standard freezing protocols may be substituted.

1.Examine and make sure the cell culture free of contamination, in healthy situation and proper confluency, etc.

2.Perform a cell count to determine the viability of cells.

3.Gently pellet the cells centrifugation (3 - 5 minutes at 1,000~2,000rpm, 4℃). Remove the supernatant using a aspirator.

4.Gently suspend the cells with CELLBANKER cryopreservation medium (1 ml for 5×10⁵ - 5×10⁶ cells).

5.Dispense the cell suspension in 1ml aliquots to cryopreservation vials that have been labeled with the cell line name, cell concentration, passage date and other essential information.

6.Place the vials directly in a -80℃ for storage. If necessary, transfer the frozen vials to a liquid nitrogen storage tank after the vials have been frozen for at least 24 hours.

IMPORTANT: Optimum protocol may change with the cell types.

Thawing

1.Remove the frozen cell from storage and quickly thaw in a 37 ℃ shaking waterbath.

2.Immediately dilute and gently mix each 1ml of cells with 10ml of complete cell culture medium.

3.Gently pellet the cells centrifugation (3-5 minutes at 1,000 - 2,000rpm, 4℃). Remove the supernatant aspirator.

4.Gently suspend the cells with appropriate volume of complete cell culture medium. And plate in a culture flask.

5.Continue the further culture procedures according to standard protocols.

Storage and Stability

Store at 2 to 8 or below -20 degrees Celsius. In unopened condition and under storage temperatures (2-8℃), the product will be stable for 3 years after the date of manufacture.

Application

Cell type	Description
293	Sheared human Ad5 DNA-transformed cell line.
293T	Human cell line expressing SV40 large T antigen
32D	Murine myeloid cell
3LL	Murine Lewis lung cancer cell
A10	Thoracic aortic smooth muscle of an embryonic rat
A2781	Glioblastoma cell
Ac2F	Rat liver cell
AtT20	Murine anterior pituitary cell
Ba/F3	Murine Pro-B cell
BHK-21	Ba Hamster renal fibroblast cell
C2C12	Mouse myoblast cell
C3H10T1/2	Murine embryonic fibroblast cell
Caco-2	Human colonic adenocarcinoma cell
CHL/1U	Neonatal Chinese Hamster Lung cell
CHO	Chinese Hamster Ovary cell
CHO-K1	Chinese Hamster Ovary cell
Colo203	Human colonic adenocarcinoma cell
COS1	African green monkey kidney cell
COS7	African green monkey kidney cell
CTLL-2	Murine T-cell
DLP-1	Human colonic adenocarcinoma cell
DT40	Chicken B lymphocyte cell
DU145	Human prostate carcinoma cell
EBV transformed B cell
EJ-1	Human bladder carcinoma
ELM-D	Murine erythroblastic leukemia cell
HeLa cell	Human Uterine Cervical Carcinoma Cell
Hep3B	Human hepatoma cell
Hepal-6	Murine hepatoma cell
Hepatocyte
HepG2	Hepatocellular carcinoma cell
HL-60	Human promyelocytic leukemia cell
HT-2	Murine cell
Huh-7	Human hepatocarcinoma cell
Jurkat	Human T-cell line
K-562	Human Caucasian Chronic myelogenous leukaemia cell
Kato-III	Human stomach carcinoma
Keratinocyte
KM12-LX	Human Caucasian Chronic myelogenous leukaemia cell
L5178Y	Murine lymphoma cell
L929	Murine fibroblast cell
LM	Murine fibroblast cell
LNCap	Human prostate adenocarcinoma cell
Lymphocyte
MCF-7	Human metastatic mammary carcinoma cell
MDCK	Madin-Dar canine kidney cell
Microvascular Endothelial Cell
Molt-4	Human T-cell
NB-1/GOTO	Human neuroblastoma
NCI-H441	Human lung adenocarcinoma epithelial cell
NIH3T3	Mouse embryonic fibroblast cell
P388	Murine leukimia cell
P3/x63-Ag8.U1	Murine myeloma cell
PC12	Rat pheochromocytoma cell
Periodontal ligament fibroblast cell
Periodontal ligament membrane fibroblast cell
Raji	Human B-cell line
RAW264.7	Murine Macrophage cell
sf9	Insect cell
SK-N-MC	Neuroepithelioma cell
SN12C	Renal carcinoma cell
SN12C	Subclone: PM6,Clone8, MM3
Stem cell	_
Stomach carcinoma cell
THle3	Human liver cell
T24	Human bladder carcinoma
Vero	African green monkey kidney epithelial cell
WEHI3B	Murine myeloid leukemia
WiDr	Human colon adenocarcinoma cell

Typical experimental results

Cells	Preservation period（year）	Viability of cells (%)
Cells	Preservation period（year）	-80℃	-196℃
MOUSE
Hybridoma	10	95	95
Myeloma	10	90	90
L929	10	90	90
FM3A	5	90	90
BALB/3T3	5	90	90
M1	5	90	90
YAC-1	5	90	-
RAT
RLC-16	5	90	90
NRK	5	90	90
PC-12	5	90	-
HAMSTER
CHO	5	90	90
V79	5	90	90
MONKEY
COS-1	5	90	90
Vero	5	90	90
HUMAN
Kidney-derived tumor cell	5	90	90
EBV transformed cell	5	90	90
HEL-derived fibroblast	5	90	90
Melanoma	5	90	90
Caco-2	3	90	-
C-5	5	90	90
CEM	5	90	90
K562	10	90	90
Jurkat	10	90	90
BALL-1	5	90	90
HUC-Fm	5	80	80