【Zenogen Pharma】CELLOTION® (Cell wash and recovery solution)

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Zenogen Pharma Zenogen CELLOTION

Description

(Cell wash and recovery solution)

CELLOTION^® is a chemically defined cell washing solution helps to increase the yield of cell recovery. The unique formulation free of serum, protein and sugars, enables for cells, tissues and organs washing.

Package:100ml/bottle

Why choose CELLOTION® Cell wash and recovery solution

A row of cell washes for each cell isolation or post-thawing manipulation may cause plenty of cell loss, particularly if the process conducted only with serum and protein-free medium. Imagine that as much as 40% of your valuable cells may go down into the drain unawarely every time after cell wash, CELLOTION® ensures you with better yields of cells.

Figure 1. Comparative Analysis of CELLOTION Cell Wash and Recovery Solution vs. General-use Cell Wash Technologies

Figure 2. Comparative Analysis (multiple washing procedures) of CELLOTION Cell Wash and Recovery Solution vs. General-use Cell Wash Technologies

Advantages

●Significantly increased cell yield after washing procedures while maintaining cell viability.

●Chemically defined solution with serum and animal derived component-free formulation.

●Simply protocol.

Application

●Stem cell isolation from a variety of sources

●Leukocyte isolation from peripheral blood

●Post-thawing cell wash

●General cell wash

Protocol

Use CELLOTION® as a substitution for general-use cell wash solution such as PBS buffer, saline or culture medium with standards cell wash procedures.

Example of use: Monocytes isolation with CELLOTION® Cell Wash and Recovery Solution.

1.Gently dilute the peripheral blood or umbilical cord blood (UCB) with CELLOTION® Cell Wash and Recovery Solution 1:1.

2.Gently add Ficoll-hypaque solution to the mixture 1:2. Do not mix and the blood should remain on top.)

3.Centrifuge the tube for 30minutes at 1,800rpm, at room temperature.

4.Collect the buffy coat (white blood cells ring) carefully with a sterile pipette tip without touching the Ficoll-hypaque solution.

5.Gently dilute the cells with CELLOTION®, then wash for 2 - 3 times in CELLOTION®.

6.Count the number of cells.

Storage and Stability

Store at 2 to 8 degrees Celsius. In unopened condition and under storage temperatures, the product will be stable for 3 years after the date of manufacture.

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