【Wako】Blotting实验级别脱脂奶粉

【Wako】Blotting实验级别脱脂奶粉

脱脂奶粉,脂肪含量低,蛋白含量高。配制成0.3~5%的水溶液使用。

用途
作为抗体反应等的印迹封闭溶液使用,也可用于干燥标本的巴氏染色前处理。

注意点
当一抗为抗磷酸化抗体时,脱脂奶粉由于含有较多磷酸化蛋白,请改为使用BSA。

应用实例

Western Blotting应用实例1
低背景!
1. 1 w/v% Skim milk in TBS-T
2. 5 w/v% BSA(Albumin, from Bovine Serum) in TBS-T

上样量:10 ug of cell lysate/Lane
抗体:Anti β-actin antibody

Western Blotting应用实例2
低背景!
1. 5w/v% Skim milk in TBS-T
2. Company A Protein-free Blocking Buffer

样品:DYKDDDDK-BAP
Lane#1:10 ng
Lane#2:5 ng
Lane#3:2.5 ng
Antibody : Anti DYKDDDDK antiboby

物性信息

外观:白色~淡黄色,粉末
溶解性:在水中变浑浊然后溶解

产品列表

※ 仅供实验研究用,不可用于临床诊断。

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