Elastin Products Company 产品列表和技术资料（六）

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EPC Elastin Products Company 是一家有着30多年悠久历史的高科技生物制品公司，为大量生物制药企业、研究院所提供各类科研级生化试剂，用于动物实验及体外测试类研究。Elastin 所有产品均供研究动物实验室使用和体外测试，禁止任何产品用作食品或药物或人类。

Elastin 产品包括琼脂糖衍生产品、抗弹性蛋白抗体、抗微纤丝蛋白抗体、抗蛋白酶抗体、生物活性肽、胶原蛋白、胶原酶及其抑制剂、金属基质蛋白酶、偶联氨基酸/抗血清、弹性蛋白酶底物、弹性蛋白肽、（人）弹性蛋白、人胰蛋白酶、人唾液白细胞酶、大/小鼠、牛胰蛋白酶、各种底物、羧肽酶等。

Elastin Products Company 产品列表

elastins

货号	名称	规格	产品描述
KE57	KE57 ETNA Elastin	250mg 500mg 1gm 3gm	Prepared from Elastin EPC No. E60 by proprietary modifications of the method of Mecham and Lange (5). Generally, bovine neck ligament elastin is extracted two times for 60 minutes at 37º C with 1 M HaOH om 80% ethanol. The second extraction supernatant is neutralized with HC103, clarified by centrifugation, dialyzed and lophilized. ETNA-elastin is a salt-free, water soluble powder.
AB415	AB415, Bovine Aorta Strips		SOURCE: Bovine Aorta. Prepared by neutral extraction method of Partridge et al. (1.) This method yields a product that is free of fat, muscle, collagen and connective tissue. This elastin is insoluble in biological buffers. Various sizes. AB415, Bovine Aorta Strips are designed to be shipped at amibient temperatures, but require special conditions and storage. Immediately upon arrival, store at the temperature indicated on the product label.
E60GI	E60GI, Elastin, Gamma Irradiated, 10 gm	10gm	Prepared by neutral extraction method. This elastin is insoluble in biological buffers. This is the starting elastin used for the preparation of all chromophore-labeled and fluorophore-labeled substrates described herein. Elastin E60 is purified from bovine neck ligament (EPC No. B214) by the neutral extraction method of partrige et. al. (1). The method yields a product that is free from fat, muscle, collagen and connective tissue. Assays of nitrogen, hodroxyproline and amino acids are used to monitor the purity during and after preparation. The final product is characterized by consistent chemical properties and optimum reactivity with elastase. It contains less than 0.01% insoluble residue after elastolysis.
RE716	RE716, Rat Lung Elastin	1mg 2mg 5mg	The rat lung elastin is purified by the method of Starcher et al. (3) in which contaminating proteins are removed by mild extraction conditions. This method minimizes peptide bond cleavage. This elastin is insoluble. The purity of the elastin is based upon the percent insoluble residue that remains after digestion with porcine elastase. Rat Lung Elastin, EPC No. RE716 contains less than 0.08% insoluble residue after elastolysis. Particle Size: Not milled; ca. 0.5-2 mm pieces.
WE99	WE99, Horse Elastin	1g 2g 5g 20g	Purified from horse neck ligament by proprietary hydrolysis method. The ligament is manually freed of gross connective tissue, fat and muscle then pulverized. The mulch is boiled in water to remove soluble proteins then boiled in alkali to remove resistant proteins and collagen. The washed insoluble mulch is hydrolyzed with elastase and further purified by cellulose chromatography and lyophilized.
PA71	PA71, Porcine Aortic Elastin	50mg 100mg	The porcine aortic elastin is purified by the method of Starcher et al. (3) in which contaminating proteins are removed by mild extraction conditions. This method minimizes peptide bond cleavage. This elastin is insoluble. The purity of the elastin is based upon the percent insoluble residue that remains after digestion with porcine elastase. Porcine Aortic Elastin, EPC No. PA71 contains less than 0.08% insoluble residue after elastolysis. Particle Size: Flocculent, long fibers ca. 0.25-10 mm pieces.
AC27	AC27, Alpha-2-Elastin	10mg 25mg 50mg	Prepared from EPC No. AE17 by gel diffusion chromatography on G100 to remove traces of beta elastin. The alpha-2-elastin is a salt-free, lyophilized water soluble powder. Molecular weight, 10 - 60 KD.
WH212	WH212, Horse Elastin Soluble	25mg 100mg 200mg 500mg	Purified from horse neck ligament by proprietary hydrolysis method. The ligament is manually freed of gross connective tissue, fat and muscle then pulverized. The mulch is boiled in water to remove soluble proteins then boiled in alkali to remove resistant proteins and collagen. The mulch is hydrolyzed in EtOH/NaOH.
SB87	SB87, Bovine Aortic Elastin	500mg 1g 2g	The bovine aorta elastin is purified by the method of Starcher et al. (3) in which contaminating proteins are removed by mild extraction conditions. This method minimizes peptide bond cleavage. This elastin is insoluble. The purity of the elastin is based upon the percent insoluble residue that remains after digestion with porcine elastase. Bovine Aortic Elastin, EPC No. SB87 contains less than 0.08% insoluble residue after elastolysis. Particle Size: Pass 100 Mesh
SC55	SC55, Canine Aortic Elastin	50mg 200mg 100mg	The canine aortic elastin is purified by the method of Starcher et al. (3) in which contaminating proteins are removed by mild extraction conditions. This method minimizes peptide bond cleavage. Thhis elastin is insoluble. The purity of the elastin is based upon the percent insoluble residue that remains after digestion with porcine elastase. Canine Aortic Elastin, EPC No. SC55 contains less than 0.08% insoluble residue after elastolysis.
SP46	SP46, Porcine Aortic Elastin	50mg 100mg 200mg 500mg	The porcine aortic elastin is purified by the method of Starcher et al. (3) in which contaminating proteins are removed by mild extraction conditions. This method minimizes peptide bond cleavage. This elastin is insoluble The purity of the elastin is based upon the percent insoluble residue that remains after digestion with porcine elastase. Porcine Aortic Elastin, EPC No. SP46 contains less than 0.08% insoluble residue after elastolysis. Particle Size: Pass 100 Mesh.
SB77	SB77, Bovine Lung Elastin	100mg 250mg 500mg	The bovine lung elastin is purified by the method of Starcher et al. (3) in which contaminating proteins are removed by mild extraction conditions. This method minimizes peptide bond cleavage. This elastin is insoluble. The purity of the elastin is based upon the percent insoluble residue that remains after digestion with porcine elastase. Bovine Lung Elastin, EPC No. SB77 contains less than 0.20% insoluble residue after elastolysis.
MT60	MT60, Hamster Lung Alpha-Elastin	1mg 2mg 5mg	Prepared from hamster lung elastin (EPC No. MT65) by extraction with oxalic acid and coacervation at pH 5 and 50° C. Soluble in water.
MT65	MT65, Hamster Lung Elastin	1mg 2mg 5mg	The hamster lung elastin is purified by the method of Starcher et al. (3) in which contaminating proteins are removed by mild extraction conditions. This method minimizes peptide bond cleavage. This elastin is insoluble. The purity of the elastin is based upon the percent insoluble residue that remains after digestion with porcine elastase. Hamster Lung Elastin, EPC No. MT65 contains less than 0.20% insoluble residue after elastolysis.
F65	F65, Elastin, bovine neck ligament	25g 50g 100g	Prepared by neutral extraction method. Flocculent, long fibers ca. 0.25 - 10 mm. Same as E60 except not milled.
K267	K267, ETNA-2-Elastin	25mg 50mg 100mg 500mg	Prepared from Etna-Elastin (KE57) by gel diffusion chromatography on Sephadex G200 (5). ETNA-2-Elastin is a salt-free, lyophilized, water soluble powder.
LK215	LK215, New Zealand White Rabbit Aortic Elastin	2mg 5mg 10mg	The New Zealand white rabbit aortic elastin is purified by the method of Starcher et al. (3) in which contaminating proteins are removed by mild extraction conditions. This method minimizes peptide bond cleavage. This elastin is insoluble. The purity of the elastin is based upon the percent insoluble residue that remains after digestion with porcine elastase. New Zealand White Rabbit Aortic Elastin, EPC No. LK215 contains less than 0.08% insoluble residue after elastolysis. Particle Size: Pass 100 Mesh.
ME15	ME15, Mouse Lung Elastin	1mg 2mg 5mg	The mouse lung elastin is purified by the method of Starcher et al. (3) in which contaminating proteins are removed by mild extraction conditions. This method minimizes peptide bond cleavage. This elastin is insoluble. The purity of the elastin is based upon the percent insoluble residue that remains after digestion with porcine elastase. Mouse Lung Elastin, EPC No. ME15 contains less than 0.08% insoluble residue after elastolysis. Particle Size: Not milled; ca. 0.5-2 mm pieces.
ES60	ES60, Elastin	5g 10g 25g	Prepared by neutral extraction method. This elastin is insoluble in biological buffers. This is the starting elastin used for the preparation of all chromophore-labeled and fluorophore-labeled substrates described herein. Elastin E60 and ES60 is purified from bovine neck ligament (EPC No. B214) by the neutral extraction method of partrige et. al. (1). The method yields a product that is free from fat, muscle, collagen and connective tissue.
ES61	ES61, Elastin, from bovine neck ligament	5g 10g 25g	Prepared by alkaline extraction method. This elastin is insoluble in biological buffers. Prepared from bovine neck ligament (EPC No. B214) by the alkaline extraction method of Lowry et al. (2). Contains less than 0.01% insoluble residue after elastolysis.
ES70	ES70, Elastin, bovine neck ligament	500mg 1g 2g 5g	Elastin, No. E70 and ES70 are prepared by the method of Starcher et al. (3) wherein the bovine neck ligament is extracted with non-degradative reagents chosen specifically to minimize peptide bond cleavage.
E60	E60, Elastin	10g 25g 50g	Prepared by neutral extraction method. This elastin is insoluble in biological buffers.
E61	E61, Elastin, from bovine neck ligament	10g 25g 50g 100g	Prepared by alkaline extraction method. This elastin is insoluble in biological buffers. Prepared from bovine neck ligament (EPC No. B214) by the alkaline extraction method of Lowry et al. (2). Contains less than 0.01% insoluble residue after elastolysis.
E70	E70, Elastin, bovine neck ligament	500mg 1g 2g 5g 10g	Elastin, No. E70 and ES70 are prepared by the method of Starcher et al. (3) wherein the bovine neck ligament is extracted with non-degradative reagents chosen specifically to minimize peptide bond cleavage.
ES12	ES12, Elastin-Soluble	0.5g 1g 2g 5g	Elastin-Soluble is prepared from Elastin EPC No. E60 by the method of Partrige et al. (1). In the method, bovine neck ligament elastin is solubilized with hot oxalic acid solution, clarified, dialyzed and lyophilized.
AE17	AE17, Alpha-Elastin	50mg 100mg 250mg	Alpha elastin is fractionated from Elastin-Soluble (EPC No. ES12) by the coacervation method of Partrige et al. (1). In the method, the coacervated alpha elastin is separated from the soluble beta elastin at pH 4.7 and 50°C. Coacervation is repeated two times more in order to improve the purity of the alpha elastin fractions. Alpha elastin has a molecular weight of 10-60 KD and runs as a smear on PAGE. Alpha Elastin is a salt-free, lyophilized, water soluble powder.
B214	B214, Bovine Neck Ligament	25g 50g 100g 500g	Fresh bovine neck ligament is freed of adhering fat, muscle and connective tissue and lyophilized. No chemicals except distilled water contact the tissue during the cleaning process. The final product is a convenient starting material for the preparation of purified elastin by chemical or neutral extraction methods. The yield of dry elastin is approximately 65%. This elastin is insoluble in biological buffers.
BE73	BE73, Beta Elastin	50mg 100mg 250mg	Beta elastin is fractionated from Elastin-Soluble (EPC No. ES12) by the coacervation method of Partrige et al. (1). In the method, the coacervated alpha elastin is separated from the soluble beta elastin at pH 4.7 and 50° C. Coacervation is repeated two times more in order to improve the purity of the beta fraction. Beta Elastin does not coacervate. Beta Elastin has a molecular weight range of 3-10 KD. Beta elastin is a salt-free, lyophilized water soluble powder.
E194S	E194S, Elastin-Remazol, suspension	10ml 100ml	Elastin-Remazol in H2O, containing 0.01% NaN3 as perservative. 200 - 400 mesh at 40 mg/ml. Abs 1 mg/ml = 0.40 at 595 nm at pH 8.8. Refer to number 11 in Methods for assay details.
RS144	RS144, ELASTIN-RHODAMINE, 400 Mesh	500mg 1g	Elastin-rhodamine is prepared from Elastin (EPC No. E60) by labeling covalently with rhodamine-B-isothiocyanate. The particle sizes passing a 400 mesh screen (smaller than 37 micron).
R144	R144, ELASTIN-RHODAMINE, 200-400 Mesh	500mg 1g 2g 5g	Elastin-rhodamine is prepared from Elastin (EPC No. E60) by labeling covalently with rhodamine-B-isothiocyanate. The particle size 200-400 mesh (75-37 micron). Soluble peptides absorb maximally at 550 nm. Fluorescence should be determined using excitation of 550 nm and emission of 570 nm
ES80	ES80, ELASTIN-FLUORESCEIN, Pass 400 Mesh	500mg 1g	Elastin-fluorescein is prepared by labeling Elastin (EPC No. E60) with fluorescein-isothiocyanate (isomer 1) and milling to particle size pass 400 mesh (Smaller than 37 micron).
HB96	HB96, Human Lung Elastin-RBB	2mg 5mg 10mg	Human lung elastin (EPC No. HS395) labeled with the chromophore remazolbrilliant blue (RBB). The RBB is bound to elastin by ether linkage and is resistant to non-specific elution. This substrate is useful for determination of specific elastolytic activity of human sputum elastase; see Methods.
HP59	HP59, Elastin Soluble-FITC	25mg 50mg 100mg	Elastin soluble, EPC No. ES12 covalently labeled with fluorescein-isothiocyanate (Isomer 1). Non-bound FITC is removed by chromatography on G25.
E80	E80, ELASTIN-FLUORESCEIN, 200-400 Mesh	500mg 1g 2g 5g	Elastin-fluorescein is prepared by labeling Elastin (EPC No. E60) with fluorescein-isothiocyanate (isomer 1) and milling to particle size 200-400 mesh (75-37 micron). Soluble peptides absorb maximally at 495 nm. Fluorescence should be determined using excitation of 490 nm and emission of 520 nm.
AE48	AE48, Alpha Elastin - FITC	25mg 50mg 100mg	Prepared from Alpha-elastin (EPC No. AE17) covalently labeled with fluorescein-isothiocyanate (Isomer 1). Excess FITC is removed from the elastin by size exclusion chromatography.
AZ25	AZ25, AZO Elastin-Soluble	50mg 100mg	Prepared from Elastin-soluble (EPC No. ES12) dyed with sulfinilic acid by the method of Tomarelli et al. (51). Absorbance of TCA-soluble peptides is 450 nm.
E164	E164, ELASTIN-CONGO RED	500mg 1g 2g 5g	Elastin-Congo Red is prepared by labeling Elastin No. E60 with the chromophore congo red by modification of the method of Hall (10). Soluble peptides absorb maximally at 485 nm. This elastin is insoluble. Particle size: 200 - 400 Mesh (75-37 Micron)