【Cosmo Bio】COS banker [Cell Freezing Medium] (chemical defined)
COS banker [Cell Freezing Medium] (chemical defined)
Catalog No. KOJ-COS-CFM01
Cos Banker is a one step culture cell storage solution. Suspend culture cells in Cos Banker and store at -80 degrees for long term storage (it is not necessary to store in liquid nitrogen). This is a serum-free solution with no animal products added.
Feature and Advantages
Ready to Use (No Dilution Required!)
Possible to store the Cells at -80℃ in Deep freezer
Possible to retain Cells in high Viability after defrosting
Does not contain Serum or Animal products*
Endotoxin-free tested / Bacteria free tested
* "Cos Banker II" contains Human Serum Albmin (GMP Grade) as Stabilizer
Components
Cos Banker (120 ml)
Contains DMSO 10%
No serum
Store at 2-8 degrees of dark place
Expiration date: 2 years from date of manufacture
Applications tested
OKT4 (mouse hybridoma cells)
PC12 (human neuronal cells)
K562 (human leukemia cells)
Other human and mouse lymphocytes
Instructions
Collect culture cells (during logarithmic growth stage) and place 5x105 - 1x107 cells in a cryovial. Centrifuge for 5 minutes at 1,200 rpm.
Add 1 ml of Cos Banker to cell pellet and vigorously mix, then freeze and store at -80°C.
If liquid Nitrogen storage is desired, freeze cells at -80°C first and then transfer to liquid Nitrogen.
To thaw, defrost in 37°C water bath quickly.
Note: It is ideal to use a logarithmic growth stage culture in order to increase the survival rate when thawed.
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