【Cosmo Bio】COS banker [Cell Freezing Medium] (chemical defined)

【Cosmo Bio】COS banker [Cell Freezing Medium] (chemical defined)

COS banker [Cell Freezing Medium] (chemical defined)


Catalog No. KOJ-COS-CFM01


Cos Banker is a one step culture cell storage solution. Suspend culture cells in Cos Banker and store at -80 degrees for long term storage (it is not necessary to store in liquid nitrogen). This is a serum-free solution with no animal products added.


Feature and Advantages

  • Ready to Use (No Dilution Required!)

  • Possible to store the Cells at -80℃ in Deep freezer

  • Possible to retain Cells in high Viability after defrosting

  • Does not contain Serum or Animal products*

  • Endotoxin-free tested / Bacteria free tested

* "Cos Banker II" contains Human Serum Albmin (GMP Grade) as Stabilizer


Components

  • Cos Banker (120 ml)

  • Contains DMSO 10%

  • No serum

Store at 2-8 degrees of dark place

Expiration date: 2 years from date of manufacture


Applications tested

  • OKT4 (mouse hybridoma cells)

  • PC12 (human neuronal cells)

  • K562 (human leukemia cells)

  • Other human and mouse lymphocytes


Instructions

  1. Collect culture cells (during logarithmic growth stage) and place 5x105 - 1x107 cells in a cryovial. Centrifuge for 5 minutes at 1,200 rpm.

  2. Add 1 ml of Cos Banker to cell pellet and vigorously mix, then freeze and store at -80°C.

  3. If liquid Nitrogen storage is desired, freeze cells at -80°C first and then transfer to liquid Nitrogen.

  4. To thaw, defrost in 37°C water bath quickly.

Note: It is ideal to use a logarithmic growth stage culture in order to increase the survival rate when thawed.


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