Elastin Products Company 产品列表和技术资料（四）

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EPC Elastin Products Company 是一家有着30多年悠久历史的高科技生物制品公司，为大量生物制药企业、研究院所提供各类科研级生化试剂，用于动物实验及体外测试类研究。Elastin 所有产品均供研究动物实验室使用和体外测试，禁止任何产品用作食品或药物或人类。

Elastin 产品包括琼脂糖衍生产品、抗弹性蛋白抗体、抗微纤丝蛋白抗体、抗蛋白酶抗体、生物活性肽、胶原蛋白、胶原酶及其抑制剂、金属基质蛋白酶、偶联氨基酸/抗血清、弹性蛋白酶底物、弹性蛋白肽、（人）弹性蛋白、人胰蛋白酶、人唾液白细胞酶、大/小鼠、牛胰蛋白酶、各种底物、羧肽酶等。

Elastin Products Company 产品列表

collagens

货号	名称	规格	产品描述
C587GI	C587GI, Collagen Type I Gamma Irradiated , 50 mg	50mg	Soluble from calf skin, Type I, Lyophilized. Gamma Irrradiatied Prepared according to the method of Gallop and Seifter (21). In the method, fresh calf skin is extracted with 0.5 M NaOAc to remove non-collagen proteins. The soluble collagen is extracted with 0.075 M sodium citrate pH 3.7 and precipitated as fibrils by dialysis against 0.02 M Na2HPO4. Soluble in 0.01 M to 0.5 M acetic acid, maximum 10 mg/ml. Soluble calf skin collagen is suitable for gel formation and assay of collagenase. Collagen is soluble in 0.075 M sodium citrate pH 3.7 and in dilute acetic acid 0.01 M to 0.5 M.
RL257	RL257, Rat Lung Collagen, 50 ugm	50ugm	Prepared from rat lung by pepsin-acid extraction, salt precipitation, re-solubilization in acetic acid and re-precipitated by dialysis against neutral phosphatae buffer. The collagen is re-solubilized and lyophilized. Salt-free. Electrophoresis in 5% SDS polyacryliamide indicates high purity with typical migration, beta (2), alpha1 and alpha2 bands.
BC715	BC715, Insoluble Collagen from calf skin, 5 gm	5gm	From bovine calf skin. Types 1,2 & 3 are removed by extraction. Insoluble in H2O and biological buffers. Particle size; pass 20 mesh.
CF339	CF339, Collagen Type II, calf articular joint, FITC, 50mg	50mg	Prepared from Collagen type II, EPC Product No. CJ385, by labeling with fluorescein-isothiocyanate (isomer 1).
MLP54	MLP54, Mouse Lung Elastin Peptides	1mg 2mg 5mg	Prepared by enzymatic hydrolysis of mouse lung elastin (EPC No. ME15) with mouse pancreatic elastase (EPC No. MS838). Residual elastase is removed by affinity chromatography on matrix-bound soy bean trypsin inhibitor. Peptides are dialyzed in 1000 MWCO tubing, passed through a sterile 0.2 micron filter then lyophilized. The peptides are salt-free, soluble in water and biological buffers. Hygroscopic.
HS333	HS333, Collagen Type III	0.5mg 5*0.5mg	Acid soluble from human skin. Prepared by modifications of Trelstad, R.L., et. al., Anal. Biochem. Vol 71, 114-118 (1976). Salt-free, lyophilized. Soluble in 0.01 M HAc and acidic buffers; maximum 5 mg/ml. Purity approximately 90% in 5%SDS-PAGE.
PC278	PC278, Collagen Type I, PEPSIN TREATED	100mg 1gm 5gm	Soluble from calf skin, Type I. PEPSIN TREATED. Prepared with modifications of the method of Gallop and Seifter (21). In the method, fresh calf skin is extracted to remove non-collagen proteins. The soluble collagen is extracted with 0. 2 M HAc pH 3.5 containing pepsin and precipitated as fibrils against 0.02 M Na2HPO4. Soluble in 0.01 M to 0.5 M acetic acid, max. 10 mg/ml. Soluble in 0.075 M sodium citrate pH 3.7 and in dilute acetic acid, 0.01 M to 0.5 M.
CP312	CP312, Collagen Type l, Porcine Corium	10mg 25mg 50mg 100mg	Soluble from porcine skin, Type I, Lyophilized. Prepared by acidic extraction, clarification, precipitation by salts and fibril formation at neutral pH. Soluble in 0.01 M to 0.5 M acetic acid, maximum 10 mg/ml.
MLC61	MLC61, Collagen, From mouse lung	0.05mg 5*0.05mg	Prepared from mouse lung by pepsin-acid extraction, salt precipitation, re-solubilization in acetic acid and re-precipitation by dialysis against neutral phosphate buffer. The collagen is re-solubilized and lyophilized. Salt-free. Electrophoresis in 5% SDS polyacrylamide indicates high purity with typical migration of gamma, beta(2), alpha 1 and alpha 2 bands.
CJ385GI	CJ385GI, Collagen Type II Gamma Irriadated, 10 mg	10mg	Collagen type II is prepared from bovine calf articular joint by modifications of published methods (22, 23, 24). SPECIFICATIONS. Chromatographically purified, salt-free, lyophilized acid soluble powder. Free of type I collagen. Contains less than 0.4% proteoglycan. Soluble in 0.01 M to 0.5 M HOAc. PREPARATION. In our method of purification, starting tissue is extracted with guanidine-HC1 to remove the bulk of proteoglycan. The residue is extracted with pepsinized acetic acid at pH 3.0 to solubilize collagens. Type II collagen is purified by fractional precipitations with NaCl, precipitations by dialysis against Na2HPO4 and column chromatography at pH 4.0 on CM-cellulose and at pH 7.5 on DEAE-cellulose to remove trace quantities of residual proteoglycans and types I and III collagens. Purity is determined by SDS-PAGE electrophoresis in a reducing gel containing 5% 2-mercaptoethanol. Type I collagen is simultaneously ran as a control. The type II collagen is free of type I as indicated by the absence of alpha-2 chains. Uronic acid assay (25) is performed using glucuronolactone as standard.
CN276GI	CN276GI, Collagen Type II, Gamma Irriadated, 10 mg	10mg	Collagen type II, EPC Product No. CN276GI, is prepared from bovine nasal septum by modifications of published methods (22, 23, 24).
MC22	MC22, COLLAGEN, TYPE II, From mouse sternum, 1mg	1mg	Collagen type II, EPC Product No. MC22 is prepared from mouse sternum by modifications of published methods (22, 23,24).
DP111	DP111, Collagen Type l, Porcine Skin	10mg 25mg 50mg 100mg	Soluble from porcine skin, Type I, Lyophilized. Prepared by acidic extraction, clarification, precipitation by salts and fibril formation at neutral pH. Soluble in 0.01 M to 0.5 M acetic acid, maximum 10 mg/ml.
CS878	CS878, Collagen Type I, from Fetal Calf Skin	25mg 50mg 100mg 250mg	Soluble, from fetal calf skin, Type I. Prepared according to Gallop and Seifter (21). Lyophilized, salt-free. Soluble in 0.01 M to 0.5 M acetic acid and acidic buffers; maximum of ca. 10 mg/ml.
DR647	DR647, Collagen Type I, from Deer Skin	25mg 50mg 100mg 250mg	Soluble, from deer skin, Type I. Prepared from the skin of the white-tailed deer, Odocoileus virginianus, by the method of Gallop and Seifter (21). Lyophilized, salt-free. Soluble in 0.01 M to 0.5 M acetic acid and acidic buffers; maximum of ca. 10 mg/ml.
HS150	HS150, Human Skin Collagen, Type I	1mg 21mg 51mg	Acid soluble from human skin. Soluble in 0.01 M to 0.5 M HAc and acidic buffers; maximum of 5 mg/ml. Purity, approximately 95% by SDS-PAGE. Note:All human donor tissues are screened for the following:Basic Serology Panel #1, RPR Syphilis Serology, ABO/Rh Typing HIV-1/HIV2 Antibody Screen, Hepatitis B Surface Antigen, Hepatitis B Core IgG/Igm Antibody, Hepatitis C Virus Antibody, HTLV-I Antibody. We accept only donors who are nonreactive to all of the above screening.
L598	L598, Collagen Fluorescein, Solubilized	2ml 10ml 25ml	Prepared from calf skin collagen (EPC Product No. C857) by labeling wth fluorescein-isothicyanate (isomer 1). Excess FITC is removed by gel filtration and sequential precipitations with NaCl and dialysis (43). 3-4 mg collagen fluorescein per ml. The collagen fluorescein is dissolved 0.1 to 4 mg/ml in 0.02 M aceitic acid at 5°C for 18 24 hours. Then collagen fluorescein is dialyzed at 5°C vs. 0.1 M Tris pH 7.5 containing 0.2 M NaCl. We follow the procedure of Baici et al., Anal. Biochem., Vol 108, page 230 (1980). Store at 5°C. DO NOT FREEZE
CP286	CP286, Proteoglycan, from Bovine Nasal Septum	5mg 10mg 25mg
CP29	CP29, Proteoglycans, from Chicken Sternum	2mg 5mg 10mg	SOURCE. Chicken Sternum Chromatographically purified in 7 M Urea on DEAE-cellulose by our modified procedure of Antonopoulos, et al. (55). Analytical chromatography on Sepharose CL-2B indicates the elution pattern as described by Antonopoulos, et al. (55). Approximately 4-5% nitrogen by Kjeldahl and 20-23% glucuronolactone by uronic acid assay (56).
CP395	CP395, Proteoglycan, from Calf Articular Joints	5mg 10mg 25mg	SOURCE. Calf Articular Joints Chromatographically purified in 7 M Urea on DEAE-cellulose by our modified procedure of Antonopoulos, et al. (55). Analytical chromatography on Sepharose CL-2B indicates the elution pattern as described by Antonopoulos, et al. (55). Approximately 4-5% nitrogen by Kjeldahl and 20-23% glucuronolactone by uronic acid assay (56).
CH783	CH783, Human Lung Collagen	1mg 2mg 5mg	Prepared from adult lung by pepsin-acid extraction, salt precipitation re-solubilization in acetic acid and re-precipitation by dialysis against neutral phosphate buffer. The collagen is re-solubilized, dialyzed and lyophilized. Salt-free.
CJ385	CJ385, COLLAGEN, TYPE II, From calf articular joints	10mg 25mg 50mg 100mg 500mg	Collagen type II, EPC Product No. CJ385, is prepared from calf articular joint cartilage by modifications of published methods (22, 23, 24).
CM206	CM206, Collagen, Achilles Tendon, Smaller than 37 Micron	1g 2g 5g	Insoluble, nondenatured, from bovine achilles tendon. Prepared according to the method of Einbinder and Schubert (19) in which tendons are extracted with Na2HPO4 solution then KCl solution, de-salted, de-fatted, washed and lyophilized. Contains less than 0.5% of insoluble matter after digestion with collagenase. Suitable as a substrate for collagenase assay in the method of Mandl (20). Collagen No.CM206, Particle size: Pass 40 Mesh (Smaller than 37 Micron)
CN276	CN276, COLLAGEN, TYPE II, From bovine nasal	25mg 50mg 100mg 500mg 10mg	Collagen type II, EPC Product No. CN276 is prepared from bovine nasal septum by modifications of published methods (22, 23,24).
C857	C857, Collagen Type I, Calf Skin Lyophilized	50mg 100mg 250mg 500mg 1gm 5gm	Soluble from calf skin, Type I, Lyophilized. Prepared according to the method of Gallop and Seifter (21). In the method, fresh calf skin is extracted with 0.5 M NaOAc to remove non-collagen proteins. The soluble collagen is extracted with 0.075 M sodium citrate pH 3.7 and precipitated as fibrils by dialysis against 0.02 M Na2HPO4. Soluble in 0.01 M to 0.5 M acetic acid, maximum 10 mg/ml. Soluble calf skin collagen is suitable for gel formation and assay of collagenase. Collagen is soluble in 0.075 M sodium citrate pH 3.7 and in dilute acetic acid 0.01 M to 0.5 M.
CF308	CF308, Collagen-FITC	1gm 2gm	Insoluble, nondenatured, from bovine achilles tendon. Prepared from Collagen EPC No.CM206 by labeling with fluorescein-isothiocyanate (isomer 1). Useful as a substrate. Solubilized peptides read at excitation 490 nm and emission 500 nm.
CH25	CH25, COLLAGEN, TYPE II, From chicken sternum	5mg 10mg 25mg 50mg	Collagen type II EPC Product NO.CH25 is prepared from chicken sternum, by modifications of published methods (22, 23,24).
C204	C204, Collagen, Achilles Tendon Smaller than 37 Micron	250mg 500mg 1gm	Insoluble, nondenatured, from bovine achilles tendon. Prepared from Collagen EPC No. CM206 by incubation in dilute buffers with pepsin, then trypsin, then elastase to remove trace amounts of contaminating protein. Contains less than 0.05% of insoluble matter after digestion with collagenase. Suitable as a substrate for collagenase activity by the method of Mandl (20). No. C204 Collagen, Achilles Tendon, Particle Size: Pass 40 Mesh (Smaller than 37 Micron)
C206	C206, Collagen, from achilles tendon	5gm 10gm 25gm 2gm	Insoluble, nondenatured, from bovine achilles tendon. Prepared according to the method of Einbinder and Schubert (19) in which tendons are extracted with Na2HPO4 solution then KCl solution, de-salted, de-fatted, washed and lyophilized. Contains less than 0.5% of insoluble matter after digestion with collagenase. Suitable as a substrate for collagenase assay in the method of Mandl (20).
C598	C598, Collagen-Fluorescein	5mg 25mg 50mg 100mg	SOURCE. Calf-skin, Soluble. Prepared from soluble calf-skin collagen (Product No. C857) by labeling with fluorescein-isothiocyanate (isomer 1). Excess FITC is removed by gel filtration and sequential precipitations with NaCl and dialysis (43). Lyophilized, salt-free.Collagen-fluorescein may be used as a substrate for the determination of collagenase activity of bacterial collagenase by quantitative fluorescent assay of lysed collagen. Excitation is 490 nm, emission is 520 nm.
C806	C806, Collagen Type I, Calf Skin Solubilized	10ml 25ml 50ml 100ml	Soluble from calf skin, Type I, Lyophilized. Prepared according to the method of Gallop and Seifter (21). In the method, fresh calf skin is extracted with 0.5 M NaOAc to remove non-collagen proteins. The soluble collagen is extracted with 0.075 M sodium citrate pH 3.7 and precipitated as fibrils by dialysis against 0.02 M Na2HPO4. Soluble in 0.01 M to 0.5 M acetic acid, maximum 10 mg/ml. Soluble calf skin collagen is suitable for gel formation and assay of collagenase. No. C806, Collagen Type I, Solubilized, 10 mg/ml of collagen per ml in 0.075 M sodium citrate pH 3.7 containing 0.01% sodium azide as preservative.