【Megazyme】提供乳制品和婴儿配方奶粉解决方案

Providing an Analytical Solution:Dairy Foods and Infant Formula

In a dairy market that is increasingly globalised and constantly changing, manufacturers continue to strive for the same goal: Quality.

Megazyme’s signature assay kits allow manufacturers to measure key parameters such as sugars, acids and carbohydrates in a convenient and cost-effective way. Researched and manufactured in-house, our products have attracted worldwide acclaim for their novel methodologies and the exceptional purity of their enzymes.

The Megazyme range features products for the measurement of important analytes in dairy products and infant formula, including：

• Lactose

• Lactic Acid

• Urea/Ammonia

• Fructan and Fructo-oligosaccharides

▍▏Measurement of Lactose in Lactose-Free and Low-Lactose Products

Final Action Status AOAC 2020.08

Megazyme’s Lactose Assay Kit addresses the key challenges of measuring lactose in low-lactose samples, producing a more accurate result - greatly reducing the overestimation that occurs with lactose assay kits offered by competitors.

The manufacturing process which breaks down the naturally present lactose in the creation of lactose-free products also results in the formation of di- and tri- saccharides. These sugars interfere with most methods of lactose measurement.

Megazyme has developed a solution.

🔹Benefits of K-LOLAC over Common Enzymatic Assays

OFFICIAL  METHOD

The first enzymatic method validated by a multi-laboratory evaluation and has been accepted as an Official Final Action Status: AOAC 2020.08.

SENSITIVE

Can quantify lactose at lower limits than all other available enzymatic assays - essential for low-lactose and lactose-free products.

EFFICIENT

First sequential enzymatic assay for lactose, i.e. reduced analyst hands-on time and increased cost-effectiveness,

SELECTIVE

Uses an exclusive enzyme which hydrolyses lactose more selectively than competing methods leading to reduced overestimation.

ACCURATE

Method includes 15 min pre-incubation step to remove high background glucose levels, allowing rapid accurate measurement in low-lactose and lactose-free products.

🔹Examples of Applications for the K-LOLAC Lactose Assay Kit

• Standard and low-lactose/lactose-free dairy products

• Milk-derived food additives

• Foods containing milk (e.g. dietetic foods, bakery products, chocolates and confectioneries)

• Recently tested Infant formula: values obtained for ‘SMA-LF’ and ‘Aptamil Lactose Free’ are in close agreement with those measured using chromatography-based methods

🔹Why choose our Lactose Assay Kit? The K-LOLAC Difference

Our new Lactose Assay Kit (K-LOLAC) joins our bestselling Lactose/Galactose Assay Kit (K-LACGAR) as part of the Megazyme range for carbohydrate analysis. The Lactose Assay Kit is specifically designed for manufacturers testing low-lactose and lactose-free foods and beverages.

a: requires a single cuvette: both determinations take place in a single cuvette

b: requires two cuvettes: one for free glucose/galactose, one for glucose/galactose released from lactose

c: Lactose over-estimation is likely to happen due to the presence of GOS and the low selectivity of the ß-galactosidase used in the assay

▍▏Measurement of Fructans and FOS in Food and Infant Formula

Fructans and fructo-oligosaccharides (FOS) are of increasing interest for manufacturers in areas as diverse as infant formula and animal feed. Fructans can serve as bulking agents, sweeteners, and as nutrients for digestive and immune health.

The potential benefits of fructan are evident for producers and consumers alike, but the accurate measurement of fructan in food and beverages presents a significant analytical challenge.

Fructan measurement made simple: the ONLY Fructan Assay Kits on the market

World exclusive: Megazyme is the only company to offer assay kits specifically for the measurement of fructan. Megazyme kits include ultra-pure components, stable reagents, simple formats and best-in-class technical support. The K-FRUC Assay Kit is recommended for use with AOAC Methods 999.03 and 2016.14. Additionally, Megazyme produces highly purified amyloglucosidase, fructanase and sucrase which are suitable for use with AOAC Method 997.08 and 2016.06.

(Ion Exchange Chromatographic Method)

1. Lyophilised amyloglucosidase

2. Fructanase (inulinase)

1. Mixture of highly purified sucrase, β-amylase, pullulanase and maltase (Bottle 1)

2. Mixture of highly purified exo-inulinase and endo-inulinase (Bottle 2)

(Enzymatic/Spectrophotometric Method)

(HPAEC-PAD) and Chinese Standard GB 5009.255-2016

1. Fructanase

2. Sucrase

Megazyme is world-renowned for producing extremely pure enzyme preparations, offering reliable results with every use.