【Yeastern】ECOS™ 10B Competent Cells, Strain: DH10B
ECOS™ 10B Competent Cells
FYE507-10VL (10 preps)
Efficiency > 5 x 10⁷ cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10⁻⁴ μg/μl)
FYE507-80VL (80 preps)
Efficiency > 5 x 10⁷ cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10⁻⁴ μg/μl)
FYE508-10VL (10 preps)
Efficiency > 2 x 10⁸ cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10⁻⁴ μg/μl)
FYE508-80VL (80 preps)
Efficiency > 2 x 10⁸ cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10⁻⁴ μg/μl)
FYE507-96WL (96 preps)
Efficiency > 5 x 10⁷ cfu/μg
50 μl/well 96 wells
Do not store the cells in liquid nitrogen!
Store at -70℃
Description
DH10B is an MC1061 derivative. This strain was designed for the propagation of large insert DNA library clones. It is used extensively, taking advantage of properties such as high DNA transformation efficiency and maintenance of large plasmids.
While DH10B has been classically reported to be galU galK, the preliminary genome sequence for DH10B indicates that DH10B is actually galE galK galU+. Genome sequence indicates that DH10B is actually deoR+. Complete genome sequence has been published.
Genotype
F¯ endA1 recA1 galE15 galK16 nupG rpsL ΔlacX74 Φ80d lacZ ΔM15 araD139 Δ(lara,leu)7697 mcrA Δ(mrr¯hsdRMS¯mcrBC) λ¯
Features
• Streptomycin resistant
• High transformation efficiency
Applications
• Suitable for genomic DNA cloning
• Allow large inserts
• Cloning of methylated cytosine or adenine containing DNA
• Blue/white screening
Efficiency
> 5 x 107 cfu/μg
Recommended for:
• Subcloning
• Genomic DNA cloning
> 2 x 108 cfu/μg
Recommended for:
• Cloning of large inserts
• Genomic DNA cloning
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