【Yeastern】ECOS™ 21 Competent Cells, Strain: BL21(DE3)
ECOS™ 21 Competent Cells
FYE207-5VL (5 preps)
Efficiency > 2 x 10⁷ cfu/μg
100 μl/vial 5 vials
Control Plasmid (pUC19) 5 μl (10⁻⁴ μg/μl)
FYE207-40VL (40 preps)
Efficiency > 2 x 10⁷ cfu/μg
100 μl/vial 40 vials
Control Plasmid (pUC19) 5 μl (10⁻⁴ μg/μl)
FYE207-96WL (96 preps)
Efficiency > 2 x 10⁷ cfu/μg
50 μl/well 96 wells
Do not store the cells in liquid nitrogen!
Store at -70℃
Description
This strain provides high levels of protein expression. This strain carries the lambda DE3 lysogen, which expresses T7 RNA polymerase from the lacUV5 promoter by isopropyl-1-thio-ß-D-galactopyranoside (IPTG) induction. The mutated rne gene (rne131) encodes a truncated RNase E enzyme that lacks the ability to degrade mRNA, resulting in an increase in mRNA stability. The transformation efficiency of BL21 is usually low, so the 6 minutes/ heat shock cold plating protocol is recommended if high efficiency is desired.
Genotype
F¯ hsdS gal (λ cIts857 ind1 Sam7 nin5 lacUV5-T7 gene l)
Features
• Lack of Lon and Omp proteases allows high-level protein expression and accumulation
• Easy gene induction
Applications
• IPTG induced gene expression in E. coli
• Ideal for high level expression of non-toxic recombinant protein expression using T7-based expression vectors
• Subcloning
Efficiency
> 2 x 107 cfu/μg
Recommended for:
• Recombinant protein expression
• Subcloning
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