【Yeastern】ECOS™ 2163 Competent Cells, Strain: GM2163
ECOS™ 2163 Competent Cells
FYE807-10VL (10 preps)
Efficiency > 1 x 10⁷ cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10⁻⁴ μg/μl)
FYE807-80VL (80 preps)
Efficiency > 1 x 10⁷ cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10⁻⁴ μg/μl)
FYE807-96WL (96 preps)
Efficiency > 1 x 10⁷ cfu/μg
50 μl/well 96 wells
Do not store the cells in liquid nitrogen!
Store at -70℃
Description
GM2163 is an E. coli K12 strain, which is deficient in both dam and dcm genes thus is suitable for the propagation of plasmid or cloned DNA to be cut with Dam or Dcm-sensitive restriction enzymes. This strain is not recommended as a host for primary cloning/ligation since the dam mutation can result in higher mutation rates and a reduction in the transformation efficiency.
Genotype
F¯ ara¯14 leuB6 thi¯1 fhuA31 lacY1 tsx¯78 galK2 galT22 supE44 rpsL136(strr) xyl¯5 mtl¯1 dam13:Tn9 (camr) dcm¯6 mcrB1 hsdR2(rk¯mk+) mcrA
Features
• Chloramphenicol resistant
• Not suitable for blue/white screening
• Not recommended as a host for primary cloning/ligation
Applications
• Propagation of plasmid free of Dam and Dcm methylations
Efficiency
> 1 x 107 cfu/μg
Recommended for:
• Propagation of plasmid to be cut with Dam or Dcm-sensitive restriction enzymes
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