【Yeastern】ECOS™ Blue Competent Cells, Strain: XL1-Blue
ECOS™ Blue Competent Cells
FYE107-10VL (10 preps)
Efficiency > 1 x 10⁸ cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10⁻⁴ μg/μl)
FYE107-80VL (80 preps)
Efficiency > 1 x 10⁸ cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10⁻⁴ μg/μl)
FYE108-10VL (10 preps)
Efficiency > 5 x 10⁸ cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10⁻⁴ μg/μl)
FYE108-80VL (80 preps)
Efficiency > 5 x 10⁸ cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10⁻⁴ μg/μl)
FYE109-10VL (10 preps)
Efficiency > 2 x 10⁹ cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10⁻⁴ μg/μl)
FYE109-80VL (80 preps)
Efficiency > 2 x 10⁹ cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10⁻⁴ μg/μl)
FYE107-96WL (96 preps)
Efficiency > 1 x 10⁸ cfu/μg
50 μl/well 96 wells
FYE108-96WL (96 preps)
Efficiency > 5 x 10⁸ cfu/μg
50 μl/well 96 wells
FYE109-96WL (96preps)
Efficiency > 2 x 10⁹ cfu/μg
50 μl/vial 96 wells
Do not store the cells in liquid nitrogen!
Store at -70℃
Description
XL1-Blue is the most popular strain for blue/white screening. It is also an excellent host strain for routine cloning application using plasmid or lambda vectors. XL1-Blue cells are endonuclease (endA) deficient, which greatly improve the quality of mini-prep DNA, and are recombination (recA) deficient, improving insert stability. The hsdR mutation prevents the cleavage of cloned DNA by the EcoK endonuclease system. The lacIqZΔM15 gene on the F´ episome allows blue-white color screening.
Genotype
F' recA1 endA1 gyrA96 thi¯1 hsdR17(rk¯, mk+) supE44 λ¯ Δ(lac) proAB lacIqZΔM15 Tn10 (tetr)
Features
• A strain modified to be ideal for blue/white screening
• Tetracycline resistant
Applications
• Routine cloning application
• Subcloning
• cDNA library
• Blue/white screening
Efficiency
> 5 x 107 cfu/μg
Recommended for:
• Routine cloning and subcloning
• Blue/white screening
> 2 x 109 cfu/μg
Recommended for:
• Routine cloning and subcloning
• Blue/white screening
> 5 x 108 cfu/μg
Recommended for:
• Routine cloning and subcloning
• Blue/white screening
• cDNA library
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