【Yeastern】ECOS™ 9-5 Competent Cells(Strain: JM109)

FYE707-10VL (10 preps)

Efficiency > 5 x 10⁷ cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10⁻⁴ μg/μl)

FYE707-80VL (80 preps)

Efficiency > 5 x 10⁷ cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10⁻⁴ μg/μl)

FYE708-10VL (10 preps)

Efficiency > 1 x 10⁸ cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10⁻⁴ μg/μl)

FYE708-80VL (80 preps)

Efficiency > 1 x 10⁸ cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10⁻⁴ μg/μl)

FYE709-10VL (10 preps)

Efficiency > 1 x 10⁹ cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10⁻⁴ μg/μl)

FYE709-80VL (80 preps)

Efficiency > 1 x 10⁹ cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10⁻⁴ μg/μl)

FYE707-96WL (96 preps)

Efficiency > 5 x 10⁷ cfu/μg
50 μl/well 96 wells

FYE708-96WL (96 preps)

Efficiency > 1 x 10⁸ cfu/μg
50 μl/well 96 wells

FYE709-96WL (96preps)

Efficiency > 1 x 10⁹ cfu/μg
50 μl/vial 96 wells

Do not store the cells in liquid nitrogen!

Store at -70℃

JM109, a K strain bacterium, carries mutations in recA and endA, leading to minimal recombination and improved quality of isolated plasmid DNA. In addition, the cells carry the F’ episome which allows blue/white screening for recombinant DNA. Thus, it has been a popular strain for routine subcloning. The cells can also be utilized for single-stranded DNA rescue when M13 or phagemid systems are used simultaneously. The JM109 strain is sensitive to all common antibiotics.

This product can become a powerful cloning tool when it is used in combination with Yeastern’s rapid ligation kit (#FYC003-100R). The ligation can be finished within 5 minutes and followed by 1-minute transformation. The fast growth rate of JM109 allows colonies to show up within 8 hours. Try this strain when you are in urgent.

F' traD36 proA+ proB+ lacIq Δ(lacZ)M15 Δ(lac-proAB) supE44 hsdR17 recA1 gyrA96 thi¯1 endA1 relA1 e14¯ λ¯

• Rapid growing strain

• Carries F' episome

• Preparation of ssDNA

• Construction of gene library

• Subcloning

> 1 x 10⁸ cfu/μg

Recommended for:

• High efficiency cloning

• Cloning low abundance transcripts

• General cloning

• Gene library

> 1 x 10⁹ cfu/μg

Recommended for:

• High efficiency cloning

• Cloning low abundance transcripts

• General cloning

• Gene library

> 5 x 10⁷ cfu/μg

Recommended for:

• Subcloning

• General cloning

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