【Yeastern】ECOS™ 101 Competent Cells（Strain: DH5α）
ECOS™ 101 Competent Cells
FYE607-10VL (10 preps)
Efficiency > 1 x 10⁸ cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10⁻⁴ μg/μl)
FYE607-80VL (80 preps)
Efficiency > 1 x 10⁸ cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10⁻⁴ μg/μl)
FYE608-10VL (10 preps)
Efficiency > 3 x 10⁸ cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10⁻⁴ μg/μl)
FYE608-80VL (80 preps)
Efficiency > 3 x 10⁸ cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10⁻⁴ μg/μl)
FYE609-10VL (10 preps)
Efficiency > 1 x 10⁹ cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10⁻⁴ μg/μl)
FYE609-80VL (80 preps)
Efficiency > 1 x 10⁹ cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10⁻⁴ μg/μl)
FYE607-96WL (96 preps)
Efficiency > 1 x 10⁸ cfu/μg
50 μl/well 96 wells
FYE608-96WL (96 preps)
Efficiency > 3 x 10⁸ cfu/μg
50 μl/well 96 wells
FYE609-96WL (96 preps)
Efficiency > 1 x 10⁹ cfu/μg
50 μl/vial 96 wells
FYE678-10VL (10 preps)
Efficiency > 5 x 10⁸ cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10⁻⁴ μg/μl)
FYE678-80VL (80 preps)
Efficiency > 5 x 10⁸ cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10⁻⁴ μg/μl)
Do not store the cells in liquid nitrogen!
Store at -70ºC
Description
DH5α is the most popular E. coli strain for everyday cloning applications. It supports blue/white screening for easy selection of recombinant DNA with X-Gal. In addition, DH5α carries recA1 mutation that eliminates homologous recombination ensuring insert stability. It also carries endA1 mutations that greatly improve the quality of plasmid DNA and yield prepared from mini-prep. It is useful for the transformation of large plasmids and two-hybrid systems (up to 14 kb).
Genotype
F¯ endA1 hsdR17(rk¯, mk¯) supE44 thi-1 λ¯recA1 gyrA96 relA1 Δ(argF-lacZYA) U169 Φ80d lacZ ΔM15 deoR
Features
Suitable for cloning with large plasmid and cDNA library construction, and also allow blue-white colony screening.
Applications
• Cloning and subcloning
• Scale-up application
• Blue/white screening
Efficiency
> 1 x 108 cfu/μg
Recommended for:
• Subcloning
• General cloning
>3 x 108 cfu/μg
Recommended for:
• High efficiency cloning
• Cloning low abundance transcripts
• General cloning
• Gene library
> 1 x 109 cfu/μg
Recommended for:
• Super high efficiency cloning
• Cloning lowest abundance transcripts
• Gene library
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