【Yeastern】Direct RT-PCR Kit

Direct RT-PCR Kit for Culture Cells

FYT506-50P

FYT506-100P

Direct cDNA Kit for Culture Cells

FYT507-50P

FYT507-100P

Direct RT-PCR Kit for Animal Tissues

FYT521-50P

Direct cDNA Kit for Animal Tissues

FYT522-50P

Direct RT-PCR Kit for Blood

FYT523-50P

Direct cDNA Kit for Blood

FYT524-50P

YB Direct RT-PCR Kits provide fast and simple procedures for preparing firststrand cDNA directly from cultured cells, animal tissues or whole blood without RNA purification. The whole process can be completed in less than 1.5 hours for cultured cells and animal tissues, and 2 hours for blood!

RNA in the lysate can be directly converted to cDNA and subsequently analyzed using the PCR reagents included in the kit or real-time RT-PCR kits purchased elsewhere. Because no removal of genomic DNA is included in the protocol, users need to design primers from the exon-exon junctions of the target mRNA to allow detection of RNA only.

• Prepare sample lysates from cultured cells or animal tissues within 10 min and from whole blood within 30 min, and use directly for RT-PCR.

• From cells or animal tissues to cDNA in only 2 steps and less than 0.5 hour.

• Sensitive in detecting low-abundance transcripts.

• Easy and efficient parallel processing of multiple samples.

• High thermostability of Deoxy+ HiSpec Reverse Transcriptase for specific and long cDNA synthesis.

• Convenient format – premixed solutions for use in RT-qPCR.

• Requires to design RNA-specific primers for detection of RNA only.

• Generation of cDNA products with high fidelity for cloning and sequencing

• Gene expression analysis

• Analysis of a large number of differentially treated cultures or animal tissues

• RT-qPCR

The performance of Direct RT-PCR Kit is tested in an RT reaction using human embryonic kidney cell lysate with B2M specific primer. The sensitivity of the kit is verified by the detection of B2M transcript after 40 cycles. The length of cDNA achieve is verified by detection of a 248 bp by DNA agarose gel and DNA sequencing.

FYT506-50P (50 preps)

Wash Buffer 25 ml
Cell Lysis Buffer 1.25 ml x 2
Deoxy+ HiSpec Reverse
Transcriptase 100 μl
2× RT Buffer 500 μl
10 μm d(T)₂₀ 50 μl
Nuclease-free Water 1 ml
RealStart DNA
Polymerase Premix 1.25 ml

FYT506-100P (100 preps)

Wash Buffer 50 ml
Cell Lysis Buffer 1.25 ml x 4
Deoxy+ HiSpec Reverse
Transcriptase 200 μl
2× RT Buffer 1 ml
10 μm d(T)₂₀ 100 μl
Nuclease-free Water 1 ml
RealStart DNA
Polymerase Premix 2.5 ml

FYT507-50P (50 preps)

Wash Buffer 25 ml
Cell Lysis Buffer 1.25 ml x 2
Deoxy+ HiSpec Reverse
Transcriptase 100 μl
2× RT Buffer 500 μl
10 μm d(T)₂₀ 50 μl
Nuclease-free Water 1 ml

FYT507-100P (100 preps)

Wash Buffer 50 ml
Cell Lysis Buffer 1.25 ml x 4
Deoxy+ HiSpec Reverse
Transcriptase 200 μl
2× RT Buffer 1 ml
10 μm d(T)₂₀ 100 μl
Nuclease-free Water 1 ml

Direct RT-PCR Kit for Blood

FYT523-50P (50 preps)

Blood Lysis Buffer I 50 ml
Blood Lysis Buffer II 1.25 ml x 2
Deoxy+ HiSpec Reverse
Transcriptase 100 μl
2× RT Buffer 0.5 ml
RT Primer (10 μM d(T)₂₀) 50 μl
Nuclease-free Water 1 ml
RealStart DNA
Polymerase Premix 1.25 ml

Direct cDNA Kit for Blood

FYT524-50P (50 preps)

Blood Lysis Buffer I 50 ml
Blood Lysis Buffer II 1.25 ml x 2
Deoxy+ HiSpec Reverse
Transcriptase 100 μl
2× RT Buffer 0.5 ml
RT Primer (10 μM d(T)₂₀) 50 μl
Nuclease-free Water 1 ml

Direct RT-PCR Kit for Animal Tissues

FYT521-50P (50 preps)

BioTake™ Tissue Puncher 1 pcs
BioTake™ Tissue Puncher
Tips (1.8 mm) 96 pcs
Tissue Lysis Buffer 1.25 ml x 2
Deoxy+ HiSpec Reverse
Transcriptase 100 μl
2× RT Buffer 0.5 ml
RT Primer (10 μM d(T)₂₀) 50 μl
Nuclease-free Water 1 ml
RealStart DNA Polymerase
Premix 1.25 ml

Direct cDNA Kit for Animal Tissues

FYT522-50P (50 preps)

BioTake™ Tissue Puncher 1 pcs
BioTake™ Tissue Puncher
Tips (1.8 mm) 96 pcs
Tissue Lysis Buffer 1.25 ml x 2
Deoxy+ HiSpec Reverse
Transcriptase 100 μl
2× RT Buffer 0.5 ml
RT Primer (10 μM d(T)₂₀) 50 μl
Nuclease-free Water 1 ml