【Yeastern】Deoxy+ Real-time TaqMan RT-PCR Kit

FYT505-50P (50 preps)

Deoxy+ Real-time Taqman   0.625 ml
RT PCR Premix, ROX
Sterilized ddH2O    1 ml

FYT505-100P (100 preps)

Deoxy+ Real-time Taqman  0.625 ml x 2
RT PCR Premix, ROX
Sterilized ddH2O  1 ml x 2

TaqMan detection system uses a designed fluorogenic probe to detect target PCR products during real-time PCR cycle. Fluorescent is detected when the quencher of probe is removed during the PCR extension cycle. Deoxy+ Realtime TaqMan RT-PCR Kit complete reverse transcription and TaqMan Real-time PCR in a single tube. Yeastern’s Deoxy+ HiSpec RT, Hotstart DNA polymerase and all the components necessary for performing TaqMan Real-time PCR from RNA template are specially prepared to ensure high specificity and high sensitivity.

• Ready-to-use master mix to allow faster setup, maximize throughput and reduce the contamination risk of real-time RT-PCR analysis

• Detection of even low copy numbers

• Excellent specificity and reproducibility with better signal to noise ratio

• Higher PCR efficiency

• Validation of siRNA-mediated gene knockdown

• Detection of multiple gene expressions

The performance of Deoxy+ Real-time TaqMan RT-PCR kit is tested in an RT reaction using human embryonic kidney cell lysate with primer d(T)20. The sensitivity of the kit is verified by the detection of B2M transcript after 40 cycles. The length of cDNA achieved is verified by detection of a 248 bp by DNA agarose gel and DNA sequencing.

FYT013-200UL

dNTPs (10 mM) 200 μl

FYT014-100UL

dCTP (100 mM) 100 μl

FYT015-100UL

dATP (100 mM) 100 μl

FYT016-100UL

dGTP (100 mM) 100 μl

FYT017-100UL

dTTP (100 mM) 100 μl

Yeastern Biotech offers nucleotides with high purity for use in PCR, RT-PCR, RT assay, DNA labeling reactions and sequencing/cycle sequencing analysis.

• Greater than 99% purity confirmed by HPLC.

• Free of trace contaminating nucleotides.

• Free of endo- and exodeoxyribonuclease, ribonuclease, phosphatase and nicking activities.

• Highly stable – the neutral pH of the nucleotide solutions ensures stability during long-term storage.

• Stable for years at -20°C.

• Stable after multiple freeze-thaw cycles.

• 90-95% of dNTPs remain in triphosphate form after 7 weeks at room temperature.

• 85-90% of dNTPs remain in triphosphate form after 30 cycles of PCR (1 min at 94°C; 3 mins at 72°C).

• Application tested in standard PCR, high fidelity PCR, long range PCR (40 kb), cDNA synthesis, RT-PCR, and real-time PCR.

Yeastern Biotech dNTPs can be used in all molecular biology applications including standard PCR, high fidelity, long PCR, LAMP-PCR, cDNA synthesis, RT-PCR, real-time PCR, RDA, MDA, DNA labeling and DNA sequencing.

• dCTP, dATP, dGTP and dTTP are all in the form of sodium salt (pH 8.3); > 99% dCTP (HPLC), < 0.9% dCDP.

• Greater than 99% purity of each component confirmed by HPLC. Functionally tested in PCR with Taq and Pfu DNA Polymerases. The absence of endo-, exodeoxyribonuclease, ribonuclease and nicking activities confirmed by appropriate tests.