【Yeastern】Deoxy+ OneStep RT-PCR Kit
Deoxy+ OneStep RT-PCR Kit
FYT503-50P (50 preps)
2× Deoxy+ OneStep RT 0.625 ml
PCR Premix
Sterilized ddH₂O 1 ml
FYT503-100P (100 preps)
2× Deoxy+ OneStep RT 0.625 ml x 2
PCR Premix
Sterilized ddH₂O 1 ml x 2
Description
The Deoxy+ OneStep RT-PCR Kit is a ready-to-use master mix, which eliminates the need for optimization of reaction and cycling conditions for one-step RT-PCR. The reaction can be prepared by simply adding template RNA and primers to the master mix. The use of Yeastern’s Hotstart DNA polymerase and Deoxy+ HiSpec RT enables reliable real-time RT-PCR quantification on any realtime PCR machines. Since it is a one-tube reaction, the procedure makes highthroughput analysis possible.
After reverse transcription, reactions are heated to 95°C for 10 minutes to inactivate the reverse transcriptase and simultaneously activate HotStart Taq DNA polymerase. This hot start to the PCR eliminates any nonspecific amplification products such as primer-dimers and reduces background smear, ensuring highly sensitive and reproducible RT-PCR.
Features
• Fast and easy one-tube setup
• One-step RT-PCR of any RNA template without optimization
• Unique enzyme mix for high specificity and sensitivity
• Optimized reverse-transcription and amplification buffer
Applications
• Onestep RT-PCR
• Gene-expression analysis
Quality Control
The performance of Deoxy+ OneStep RT-PCR Kit is tested in an RT reaction using human embryonic kidney cell lysate with primer d(T)20. The sensitivity of the kit is verified by the detection of B2M transcript after 40 cycles. The length of cDNA achieved is verified by detection of a 248 bp by DNA agarose gel and DNA sequencing.
Deoxy+ Real-time SYBR Green RT-PCR Kit
FYT504-50P (50 preps)
2× Deoxy+ Real-time SYBR 0.625 ml
Green RT PCR Premix, ROX
Sterilized ddH₂O 1 ml
FYT504-100P (100 preps)
2× Deoxy+ Real-time SYBR 0.625 ml x 2
Green RT PCR Premix, ROX
Sterilized ddH₂O 1 ml x 2
Description
Deoxy+ Real-time SYBR Green RT-PCR system provides users with a rapid and simple way to quantify the expression of gene of interest based on Real-time PCR system containing SYBR Green. Yeastern’s Deoxy+ HiSpec RT, Hotstart DNA polymerase and all the components for Real-time SYBR Green RT-PCR are skillfully mixed within a single tube. Unique buffer system allows highly specific quantification by preventing the formation of nonspecific products and primer–dimers.
Features
• Ready-to-use master mix to allow faster setup, maximize throughput and reduce the contamination risk of real-time RT-PCR analysis
• Detection of even low copy numbers
• Excellent sensitivity and easy optimization with SYBR Green
Applications
• Validation of siRNA-mediated gene knockdown
• Detection of gene regulation
Quality Control
The performance of Deoxy+ Real-time SYBR Green RT-PCR Kit is tested in an RT reaction using human embryonic kidney cell lysate with primer d(T)20. The sensitivity of the kit is verified by the detection of B2M transcript after 40 cycles. The length of cDNA achieved is verified by detection of a 248 bp by DNA agarose gel and DNA sequencing.
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