【Yeastern】Deoxy+ HiSpec Reverse Transcriptase
Deoxy+ HiSpec Reverse Transcriptase
FYT501-100R (100 rxns)
Deoxy+ HiSpec Reverse 50 μl x 2
Transcriptase (20000 U)
2× Deoxy+ RT Premix* 0.5 ml x 2
(* 2× Deoxy+ RT Premix contains RT buffer,
0.1 M DTT and 10 mM dNTPs)
Description
Deoxy+ HiSpec Reverse Transcriptase (RT) is genetically engineered by introducing of point mutations to MMLV RT that increase half-life, reduce RNase activity and increase thermal stability. Those designed mutations lead to increased specificity of Deoxy+ HiSpec RT and the highest cDNA yield of all RTs. It is ideal for RT-PCR of a specific gene or generating cDNA from total or poly (A)+ RNA samples. It synthesizes a complementary DNA strand from total RNA, mRNA, or an RNA:DNA hybrid.
Features
• Half life of 100 minutes at 50°C for the highest cDNA yields
• Reduced RNase H activity for more full-length cDNA
• Full activity at 50°C for increased specificity with GSP
• Ability to increase RT units without inhibiting subsequent PCR
Applications
• Synthesis of first-strand cDNA
• Array labeling
• cDNA libraries
• RT-PCR, primer extension, and 3’ and 5’ RACE
Unit Definition
One unit incorporates 1 nmole of dTTP into acid precipitable material in 10 minutes at 37ºC using poly(A)-oligo(dT) as template primer.
Quality Control
This product has passed the following quality control assays: SDS-polyacrylamide gelanalysis for purity ; funct ion alabsence of endodeoxyribonuclease, 3’ and 5’ exodeoxyribonuclease, and ribonuclease activities; yield and length of cDNA product.
点在看，传递你的品味
