【Yeastern】EtB“Out” Nucleic Acid Staining Solution
EtB“Out” Nucleic Acid Staining Solution
FYD007-200P (200 preps)
EtB"Out" Nucleic Acid 1 ml
Staining Solution (20,000×)
Description
EtB“Out” Nucleic Acid Staining Solution aims to replace traditional EtBr (ethidium bromide) in performing nucleic acid detection in agarose gels. EtBr has long been known as a strong mutagen, however EtB“Out” causes only neglectable mutations in the Ames test.
The sensitivity of EtB“Out” is identical to that of EtBr. Under UV light, EtB“Out” emits green fluorescence when bound to DNA or RNA. EtB“Out” can be excited at 309 nm, 419 nm and 514 nm. The fluorescence emission peak of EtB“Out” when bound to DNA is at 500 nm.
Features
• Economic : use only 5 μl in 100 ml of agarose gel
• Sensitive : sensitivity is comparable and even better than EtBr, DNA concentration as low as 5 ng can be detecded
• Safe : non-mutagenic, non-toxic, non carcinogenic
• Green : no hazardous waste
Applications
Nucleic acids detection (dsDNA and ssRNA) within agarose gel after electrophoresis under UV illumination.
Quality Assurance
The quality of the EtB“Out” Nucleic Acid Staining Solution is tested on a lot-to-lot basis. Nucleic acids is extracted from tissues and serial diluted. Electrophoresis is performed and the agarose gel is stained with EtB“Out” Nucleic Acid Staining Solution to ensure the performance.
Results
Nucleic staining using EtB“Out” Nucleic Acid Staining Solution.Two individual agarose gels were prepared with 5 μl of EtBr and EtB“Out” respectively. Staining results were examined under UV illumination. Identical results were observed and EtB“Out” even showed a better staining result than using EtBr.
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