【Yeastern】HiYield Gel/PCR DNA Fragments Extraction Kit 2.0
HiYield Gel/PCR DNA Fragments Extraction Kit 2.0
FYG206 - 100（100preps）
EZ Buffer：60 ml
W1 Buffer：45 ml
W2 Buffer：15 ml
Elution Buffer：10 ml
EZ Column：100 pcs
Collection Tube：100 pcs
FYG206 - 300（300 preps）
EZ Buffer：80 ml x2
W1 Buffer：125 ml
W2 Buffer：25 ml x2
Elution Buffer：30 ml
EZ Column：300 pcs
Collection Tube：300 pcs
Description
The HiYield Gel/PCR DNA Fragments Extraction Kit 2.0 is designed to recover or concentrate DNA fragments (50 bp-10 kb) from agarose gels in 20 mins, PCR or other enzymatic reactions. The unique dual purpose application and high yield mini columns make this kit valuable. The method uses a chaotropic salt, guanidine thiocyanante to dissolve agarose gel and denature enzymes. DNA fragments in chaotropic salt solution bind to the glass fiber matrix of the spin column. Following washing off contaminants, the purified DNA fragments (Effective Binding Capacity Approx: 20 ug ) are eluted by addition of low salt elution buffer or water. Salts, enzymes and unincorporated nucleotides are effectively removed from reaction mixtures without phenol extraction or alcohol precipitation.
Features
•Recovery : Up to 95%
•Operation time : within 20 minutess
Applications
Fluorescent/radioactive sequencing, PCR, restriction enzyme digestion, DNA labeling and ligation
Genomic DNA Extraction Mini Kit (Blood and Urine)
FYG109 - 100 (100 preps)
B1 Buffer: 100 ml
B2 Buffer: 35 ml
B3 Buffer: 12 ml
BC Buffer: 45 ml
W1 Buffer: 45 ml
W2 Buffer: 15 ml
Elution Buffer: 10 ml
BZ Column: 100 pcs
Collection Tube: 100 pcs
Description
The Genomic DNA Extraction Mini Kit (Blood and Urine) is designed for rapid extraction of pure genomic DNA from Bacteria and fungus cell. Efficiently remove cellular debris and inhibitors, this kit using column-type tube in purification process through three simple steps of binding, washing and then elution for the safe and convenient extraction of high-purity genomic DNA. The entire process can be completed in less than 1 hour without phenol/chloroform, and the final product can be used in PCR or other downstream experiments.
Features
• Format : spin column (centrifuge)
• Yield : up to 50 μg
Sample
3109 Bacteria, 5 x 107 Fungus cells
Applications
PCR, AFLP/PADP, RFLP, Southern blot, real-time PCR
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