【Yeastern】HiYield Plasmid Mini Kit 2.0
HiYield Plasmid Mini Kit 2.0
# FYG007-100P (100 preps)
PDX1 Buffer 24 ml
PD2 Buffer 30 ml
PDX3 Buffer 40 ml
WX1 Buffer 60 ml
Wash Buffer 20 ml
Elution Buffer 10 ml
RNase A (20 mg/ml) 84 μl
PDX Column 100 pcs
2 ml Collection Tube 100 pcs
# FYG007-300P (300 preps)
PDX1 Buffer 72 ml
PD2 Buffer 90 ml
PDX3 Buffer 120 ml
WX1 Buffer 180 ml
Wash Buffer 60 ml
Elution Buffer 30 ml
RNase A (20 mg/ml) 252 μl
PDX Column 300 pcs
2 ml Collection Tube 300 pcs
Description
HiYield Plasmid Mini Kit 2.0 is specially designed for rapid isolation of plasmid or cosmid DNA from 1-5 ml of bacterial cultured cells. As high as 40 μg of high quality plasmid DNA can be purified in less than 30 minutes and is ready for restriction digestion, ligation, PCR, and sequencing reaction.
No phenol extraction or alcohol precipitation is required in this protocol. In the process, clear and extra pure cell lysate with minimal genomic DNA and RNA contaminants can be obtained through the modified alkaline lysis method and RNase treatment. In the presence of a chaotropic salt, the plasmid DNA within the lysate will then bind to the glass fiber matrix equipped in the spin column. The contaminants are washed away with an ethanol-containing wash buffer. Finally, the purified plasmid DNA is eluted by a low salt elution buffer or distilled water. Typical yields of high-purity are 20~40 μg for high-copy number plasmids or 3~10 μg for low-copy number plasmids.
Features
• Sample : 1-5 ml of bacterial cells
• Format : spin column (centrifuge)
• Yield : up to 40 μg of plasmid/cosmid DNA
• Operation time : up to 30 minutes
• Elution volume : 30-50 μl
Applications
Restriction enzyme digestion, library screening, ligation, PCR, transformation/ sequencing reactions
Quality Control
The quality of HiYield Plasmid Mini Kit 2.0 is tested on a lot-to-lot basis. The kit is tested by isolation of plasmid DNA from 5 ml culture of E. coli DH5α transformed with the plasmid pGAD424 (A600>2 units/ml). More than 40 μg of plasmid DNA should be obtained. One μg of the purified product is also tested for restriction enzyme digested with EcoR I followed by agarose gel analysis.
Results
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