【PluriSelect】细胞分选：pluriBeads-Positive Cell Separation
pluriBeads-Positive Cell Separation
为何使用pluriBeads®分选细胞？
●分选出的细胞是不带磁珠的，可用于另一种细胞的阳性或阴性富集；
●样本可以直接用全血，未结合的细胞尤其是血小板或红细胞的污染非常低；
●阳选速度更快，扩展性更小，纯度更高。
分选步骤
1、原材料准备
将样本（全血，或其他细胞悬液）和带有抗体的pluribeads混合。
2.孵育
用pluriPlix混匀器将样本和pluribeads在室温下轻轻混匀，使目的细胞可以结合到pluribeads上。
3.洗涤
将结合pluribeads的细胞过筛网，携带pluriBeads的目的细胞>筛网直径会留在筛网上面，其他细胞<筛网直径会被洗掉。
4.解离
将结合了pluribeads的目的细胞再次过筛网，用解离液冲洗，直径大的pluriBeads留在筛网上，直径小的细胞通过了筛网，从而得到没有beads的纯净细胞！
两种beads:S beads和M beads
pluriBead分选细胞的活性
经过beads分选的细胞，用Calcein AM染活细胞（绿色），碘化丙啶PI染死细胞(红色)。
结果显示：大部分都是活细胞，细胞活性很好。
四种试剂盒
产品货号：
Cat#70-50010-11
Cat#70-50010-12
Cat#70-50010-21
Cat#70-50010-22
分选范围
分选可分选种属：人、小鼠、猪、大鼠、绵羊、山羊、兔子、牛
可分选样本：白细胞层、全血、组织液、PBMC、培养的细胞系
可分选细胞：T细胞、T辅助细胞、毒性T细胞、B细胞、浆细胞、NK细胞、胸腺细胞、单核细胞、血小板，粒细胞（中性粒细胞、嗜碱性粒细胞、嗜酸性粒细胞）、白细胞、巨噬细胞、内皮细胞、红细胞、造血细胞、非造血细胞、凋亡细胞、造血祖细胞、间充质细胞、雪旺细胞、干细胞/基质细胞等大多数组织细胞。
种属 |
分选细胞 |
产品 |
货号 |
大鼠 |
单核细胞(亚群)，T辅助细胞 |
CD4 S-pluriBead® anti-rat |
cat#22-00400-10 |
CD4 M-pluriBead® anti-rat |
cat#22-00400-20 |
||
毒性T细胞，胸腺细胞 |
CD8b S-pluriBead® anti-rat |
cat#22-008b0-10 |
|
CD8b M-pluriBead® anti-rat |
cat#22-008b0-20 |
||
单核细胞，粒细胞，巨噬细胞，NK细胞（亚羊） |
CD11b S-pluriBead® anti-rat |
cat#22-011b0-10 |
|
CD11b M-pluriBead® anti-rat |
cat#22-011b0-20 |
||
白细胞，大多数组织细胞 |
CD44 S-pluriBead® anti-rat |
cat#22-04400-10 |
|
CD44 M-pluriBead® anti-rat |
cat#22-04400-20 |
||
白细胞 |
CD45 S-pluriBead® anti-rat |
cat#22-04500-10 |
|
CD45 M-pluriBead® anti-rat |
cat#22-04500-20 |
||
白细胞，大多数组织细胞 |
CD55 S-pluriBead® anti-rat |
cat#22-05500-10 |
|
CD55 M-pluriBead® anti-rat |
cat#22-05500-20 |
||
T细胞 |
Anti TCRAB S-pluriBead® anti-rat |
cat#22-TCRAB-10 |
|
Anti TCRAB M-pluriBead® anti-rat |
cat#22-TCRAB-20 |
||
任何细胞 |
universal S-pluriBead® anti-rat |
cat#31-GTaRT-10 |
|
universal M-pluriBead® anti-rat |
cat#31-GTaRT-20 |
||
小鼠 |
T细胞，胸腺细胞 |
CD3 S-pluriBead® anti-ms |
cat#29-00300-10 |
CD3 M-pluriBead® anti-ms |
cat#29-00300-20 |
||
单核细胞（亚群），T辅助细胞 |
CD4 S-pluriBead® anti-ms |
cat#29-00400-10 |
|
CD4 M-pluriBead® anti-ms |
cat#29-00400-20 |
||
NK细胞（亚群），毒性T细胞 |
CD8 S-pluriBead® anti-ms |
cat#29-00800-10 |
|
CD8 M-pluriBead® anti-ms |
cat#29-00800-20 |
||
单核细胞、粒细胞、巨噬细胞、NK细胞（亚群） |
CD11b S-pluriBead® anti-ms |
cat#29-011b0-10 |
|
CDl1b M-pluriBead® anti-ms |
cat#29-011b0-20 |
||
活化的T细胞，树突状细胞 |
CDllc S-pluriBead® anti-ms |
cat#29-011c0-10 |
|
CDllc M-pluriBead® anti-ms |
cat#29-011c0-20 |
||
单核细胞 |
CD14 S-pluriBead® anti-ms |
cat#29-01400-10 |
|
CD14 M-pluriBead® anti-ms |
cat#29-01400-20 |
||
B细胞 |
CD19 S-pluriBead® anti-ms |
cat#29-01900-10 |
|
CD19 M-pluriBead® anti-ms |
cat#29-01900-20 |
||
白细胞 |
CD31 S-pluriBead® anti-ms |
cat#29-03100-10 |
|
CD31 M-pluriBead® anti-ms |
cat#29-03100-20 |
||
循环肿瘤细胞，上皮细胞 |
CD326 S-pluriBead anti-ms |
cat#29-32600-10 |
|
CD326 M-pluriBead® anti-ms |
cat#29-32600-20 |
||
单核细胞，巨噬细胞 |
F4/80 S-pluriBead® anti-ms |
cat#29-F4800-10 |
|
F4/80 M-pluriBead® anti-ms |
cat#29-F4800-20 |
||
粒细胞 |
Ly6G S-pluriBead® anti-ms |
cat#29-Ly6G0-10 |
|
Ly6G M-pluriBead® anti-ms |
cat#29-Ly6G0-20 |
||
任何细胞 |
universal S-pluriBead® anti-ms |
cat#31-GTaMS-10 |
|
universal M-pluriBead® anti-ms |
cat#31-GTaMS-20 |
||
人 |
T细胞，NK细胞 |
CD2 S-pluriBead® anti-hu |
cat#19-00200-10 |
CD2 M-pluriBead® anti-hu |
cat#19-00200-20 |
||
T细胞，胸腺细胞 |
CD3 S-pluriBead® anti-hu |
cat#19-00300-10 |
|
CD3 M-pluriBead® anti-hu |
cat#19-00300-20 |
||
单核细胞，T辅助细胞 |
CD4 S-pluriBead® anti-hu |
cat#19-00400-10 |
|
CD4 M-pluriBead® anti-hu |
cat#19-00400-20 |
||
T细胞，B细胞，胸腺细胞 |
CD5 S-pluriBead® anti-hu |
cat#19-00500-10 |
|
CD5 M-pluriBead® anti-hu |
cat#19-00500-20 |
||
NK细胞，T细胞，胸腺细胞 |
CD7 S-pluriBead® anti-hu |
cat#19-00700-10 |
|
CD7 M-pluriBead® anti-hu |
cat#19-00700-20 |
||
NK细胞，毒性T细胞 |
CD8 S-pluriBead® anti-hu |
cat#19-00800-10 |
|
CD8 M-pluriBead® anti-hu |
cat#19-00800-20 |
||
血小板，粒细胞 |
CD9 S-pluriBead® anti-hu |
cat#19-00900-10 |
|
CD9 M-pluriBead® anti-hu |
cat#19-00900-20 |
||
白细胞 |
CDlla S-pluriBead® anti-hu |
cat#19-01la0-10 |
|
CDlla M-pluriBead® anti-hu |
cat#19-011a0-20 |
||
单核细胞，粒细胞，巨噬细胞，NK（亚群） |
CD11b S-pluriBead® anti-hu |
cat#19-011b0-10 |
|
CD11b S-pluriBead® anti-hu |
cat#19-011b0-10 |
||
单核细胞 |
CD14 S-pluriBead® anti-hu |
cat#19-01400-10 |
|
CD14 M-pluriBead® anti-hu |
cat#19-01400-20 |
||
粒细胞 |
CD15 S-pluriBead® anti-hu |
cat#19-01500-10 |
|
CD15 M-pluriBead® anti-hu |
cat#19-01500-20 |
||
中性粒细胞 |
CD16 S-pluriBead® anti-hu |
cat#19-01600-10 |
|
CD16 M-pluriBead® anti-hu |
cat#19-01600-20 |
||
B细胞 |
CD19 S-pluriBead® anti-hu |
cat#19-01900-10 |
|
CD19 M-pluriBead® anti-hu |
cat#19-01900-20 |
||
CD21 S-pluriBead® anti-hu |
cat#19-02100-10 |
||
CD21 M-pluriBead® anti-hu |
cat#19-02100-20 |
||
单核细胞，T细胞，粒细胞 |
CD31 S-pluriBead® anti-hu |
cat#19-02100-10 |
|
CD31 M-pluriBead® anti-hu |
cat#19-02100-20 |
||
白细胞，大多数组织细胞 |
CD44 S-pluriBead® anti-hu |
cat#19-04400-10 |
|
CD44 M-pluriBead® anti-hu |
cat#19-04400-20 |
||
白细胞 |
CD45 S-pluriBead® anti-hu |
cat#19-04500-10 |
|
CD45 M-pluriBead® anti-hu |
cat#19-04500-20 |
||
血小板，白细胞，红细胞 |
CD47 S-pluriBead® anti-hu |
cat#19-04700-10 |
|
CD47 M-pluriBead® anti-hu |
cat#19-04700-20 |
||
造血细胞，非造血细胞 |
CD55 S-pluriBead® anti-hu |
cat#19-05500-10 |
|
CD55 M-pluriBead® anti-hu |
cat#19-05500-20 |
||
凋亡细胞 |
CD95 S-pluriBead® anti-hu |
cat#19-09500-10 |
|
CD95 M-pluriBead® anti-hu |
cat#19-09500-20 |
||
造血祖细胞 |
CD117 S-pluriBead® anti-hu |
cat#19-11700-10 |
|
CD117 M-pluriBead® anti-hu |
cat#19-11700-20 |
||
浆细胞 |
CD138 S-pluriBead® anti-hu |
cat#19-13800-10 |
|
CD138 M-pluriBead® anti-hu |
cat#19-13800-20 |
||
嗜碱性粒细胞，嗜酸性粒细胞 |
CD193 S-pluriBead® anti-hu |
cat#19-19300-10 |
|
CD193 M-pluriBead® anti-hu |
cat#19-19300-20 |
||
红细胞 |
CD235a S-pluriBead® anti-hu |
cat#19-235a0-10 |
|
CD235a M-pluriBead® anti-hu |
cat#19-235a0-20 |
||
间充质细胞，雪旺细胞，干细胞/基质细胞 |
CD271 S-pluriBead® anti-hu |
cat#19-27100-10 |
|
CD271 M-pluriBead® anti-hu |
cat#19-11700-20 |
||
循环肿瘤细胞，上皮细胞 |
CD326 S-pluriBead® anti-hu |
cat#19-32600-10 |
|
CD326 M-pluriBead® anti-hu |
cat#19-32600-20 |
||
任何细胞 |
universal S-pluriBead® anti-hu |
cat#31-RBaHU-10 |
|
universal M-pluriBead® anti-hu |
cat#31-RBaHU-20 |
||
牛 |
血小板，粒细胞 |
CD9 S-pluriBead® anti-bovine |
cat#25-00900-10 |
CD9 M-pluriBead® anti-bovine |
cat#25-00900-20 |
||
单核细胞 |
CD14 S-pluriBead® anti-bovine |
cat#25-01400-10 |
|
CD14 M-pluriBead® anti-bovine |
cat#25-01400-20 |
||
B细胞 |
CD21 S-pluriBead® anti-bovine |
cat#25-02100-10 |
|
CD21 M-pluriBead® anti-bovine |
cat#25-02100-20 |
||
白细胞 |
CD45R S-pluriBead® anti-bovine |
cat#25-045R0-10 |
|
CD45R M-pluriBead® anti-bovine |
cat#25-045R0-20 |
||
血小板，白细胞，红细胞 |
CD47 S-pluriBead® anti-bovine |
cat#25-04700-10 |
|
CD47 M-pluriBead® anti-bovine |
cat#25-04700-20 |
||
单核细胞，T细胞，粒细胞，B细胞 |
CD62L S-pluriBead® anti-bovine |
cat#25-062L0-10 |
|
CD62L M-pluriBead® anti-bovine |
cat#25-062L0-20 |
||
造血祖细胞 |
CD117 S-pluriBead® anti-bovine |
cat#25-11700-10 |
|
CD117 M-pluriBead® anti-bovine |
cat#25-11700-20 |
||
抗原递呈细胞 |
HLARP S-pluriBead® anti-bovine |
cat#25-HLARP-10 |
|
HLARP M-pluriBead® anti-bovine |
cat#25-HLARP-20 |
||
猪 |
单核细胞、粒细胞、巨噬细胞、NK细胞（亚君群） |
CD11b S-pluriBead® anti-porcine |
cat#23-011b0-10 |
CDllb M-pluriBead® anti-porcine |
cat#23-011b0-20 |
||
单核细胞 |
CD14 S-pluriBead® anti-porcine |
cat#23-01400-10 |
|
CD14 M-pluriBead® anti-porcine |
cat#23-01400-20 |
||
B细胞 |
CD21 S-pluriBead® anti-porcine |
cat#23-02100-10 |
|
CD21 M-pluriBead® anti-porcine |
cat#23-02100-20 |
||
血小板，T细胞，内皮细胞 |
CD29 S-pluriBead® anti-porcine |
cat#23-02900-10 |
|
CD29 M-pluriBead® anti-porcine |
cat#23-02900-20 |
||
白细胞，大多数组织细胞 |
CD44 S-pluriBead® anti-porcine |
cat#23-04400-10 |
|
CD44 M-pluriBead® anti-porcine |
cat#23-04400-20 |
||
血小板，白细胞，红细胞 |
CD47 S-pluriBead® anti-porcine |
cat#23-04700-10 |
|
CD47 M-pluriBead® anti-porcine |
cat#23-04700-20 |
||
单核细胞，T细胞，粒细胞，B细胞 |
CD62L S-pluriBead® anti-porcine |
cat#23-062L0-10 |
|
CD62L M-pluriBead® anti-porcine |
cat#23-062L0-20 |
||
NK细胞，胸腺细胞 |
CD90 S-pluriBead® anti-porcine |
cat#23-09000-10 |
|
CD90 M-pluriBead® anti-porcine |
cat#23-09000-20 |
||
抗原递呈细胞 |
HLARP S-pluriBead® anti-porcine |
cat#23-HLARP-10 |
|
HLARP M-pluriBead® anti-porcine |
cat#23-HLARP-20 |
||
红细胞 |
RBCL S-pluriBead® anti-porcine |
cat#23-RBC00-10 |
|
RBC M-pluriBead® anti-porcine |
cat#23-RBCO0-20 |
||
绵羊 |
血小板，T细胞，内皮细胞 |
CD29 S-pluriBead® anti-sheep |
cat#24-02900-10 |
CD29 M-pluriBead® anti-sheep |
cat#24-02900-20 |
||
血小板，巨核细胞 |
CD41/61 S-pluriBead® anti-sheep |
cat#24-04100-10 |
|
CD41/61 M-pluriBead® anti-sheep |
cat#24-04100-20 |
||
白细胞，大多数组织细胞 |
CD44 S-pluriBead® anti-sheep |
cat#24-04400-10 |
|
CD44 M-pluriBead® anti-sheep |
cat#24-04400-20 |
||
白细胞 |
CD45 S-pluriBead® anti-sheep |
cat#24-04500-10 |
|
CD45 M-pluriBead® anti-sheep |
cat#24-04500-20 |
||
血小板，白细胞，红细胞 |
CD47 S-pluriBead® anti-sheep |
cat#24-04700-10 |
|
CD47 M-pluriBead® anti-sheep |
cat#24-04700-20 |
||
单核细胞，T细胞，粒细胞，B细胞 |
CD62L S-pluriBead® anti-sheep |
cat#24-062L0-10 |
|
CD62L M-pluriBead® anti-sheep |
cat#24-062L0-20 |
||
间充质细胞，雪旺细胞，干细胞/基质细胞 |
CD271 S-pluriBead® anti-sheep |
cat#24-27100-10 |
|
CD271 M-pluriBead® anti-sheep |
cat#24-27100-20 |
||
抗原递呈细胞 |
HLARP S-pluriBead® anti-sheep |
cat#24-HLARP-10 |
|
HLARP M-pluriBead® anti-sheep |
cat#24-HLARP-20 |
||
犬 |
单核细胞（亚群），T辅助细胞 |
CD4 S-pluriBead® anti-canine |
cat#27-00400-10 |
CD4 M-pluriBead® anti-canine |
cat#27-00400-20 |
||
血小板，T细胞，内皮细胞 |
CD29 S-pluriBead® anti-canine |
cat#27-02900-10 |
|
CD29 M-pluriBead® anti-canine |
cat#27-02900-20 |
||
山羊 |
任何细胞 |
universal S-pluriBead® anti-goat |
cat#31-RBaGT-10 |
universal M-pluriBead® anti-goat |
cat#31-31-RBaGT-20 |
||
仓鼠 |
任何细胞 |
universal S-pluriBead® anti-hamster |
cat#31-GTaHS-10 |
universal M-pluriBead® anti-hamster |
cat#31-GTaHS-20 |
||
马 |
白细胞 |
CDlla S-pluriBead® anti-horse |
cat#32-011a0-10 |
CDlla M-pluriBead® anti-horse |
cat#32-01la0-20 |
||
血小板，巨核细胞 |
CD41/61 S-pluriBead® anti-horse |
cat#32-04100-10 |
|
CD41/61 M-pluriBead® anti-horse |
cat#31-32-04100-20 |
||
兔子 |
单核细胞 |
CD14 S-pluriBead® anti-rabbit |
cat#33-01400-10 |
CD14 M-pluriBead® anti-rabbit |
cat#33-01400-20 |
||
单核细胞，T细胞，B细胞 |
CD81 S-pluriBead® anti-rabbit |
cat#33-08100-10 |
|
CD81 M-pluriBead® anti-rabbit |
cat#33-08100-20 |
||
任何细胞 |
universal S-pluriBead® anti-rabbit |
cat#31-GTaRB-10 |
|
universal M-pluriBead® anti-rabbit |
cat#31-GTaRB-20 |
实例展示
实例1.pluriBead®阳性分离单核细胞，用于分化成熟的DC细胞（需要试剂见表格）
产品名称 |
货号 |
CD14 S-pluriBead® anti-hu |
Cat#19-01400-10 |
CD14 M-pluriBead® anti-hu |
Cat#19-01400-20 |
S-pluriBead® Mini Reagent Kit |
Cat#70-50010-11 |
M-pluriBead® Mini Reagent kit |
Cat#70-50010-22 |
pluriPlix |
Cat#50-01010-80 |
实例2.B细胞的分选（所需试剂见下表）
产品名称 |
货号 |
CD19 S-pluriBead® anti-hu |
Cat#19-01900-10 |
S-pluriBead® Mini Reagent Kit |
Cat#70-50010-11 |
pluriPlix |
Cat#50-01010-80 |
实例3.pluriBeads®CD8+T细胞的分选
其他细胞品类
别划走，精彩继续
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