AAT Bioquest®：细胞凋亡相关产品
细胞凋亡
AAT 提供由凋亡早期到晚期的一系列检测探针。
▍▏caspase 相关检测
Caspase 3/7
Product Name |
Cat. # |
Ex/Em（nm） |
Size |
Amplite™ Fluorimetric Caspase 3/7 Assay Kit |
13502 |
351/430（蓝色） |
500tests |
13503 |
498/520（绿色） |
500tests |
|
13504 |
534/619（红色） |
100tests |
|
Cell Meter™ Caspase 3/7 Activity Apoptosis Assay Kit |
22795 |
351/430（蓝色） |
200tests |
22796 |
498/520（绿色） |
200tests |
Caspase 8
Product Name |
Cat. # |
Ex/Em（nm） |
Size |
Cell Meter™ Caspase 8 Activity Apoptosis Assay Kit |
22812 |
351/430（蓝色） |
200tests |
22798 |
498/520（绿色） |
200tests |
|
22816 |
534/619（红色） |
100tests |
Caspase 9
Product Name |
Cat. # |
Ex/Em（nm） |
Size |
Cell Meter™ Caspase 9 Activity Apoptosis Assay Kit |
22813 |
351/430（蓝色） |
200tests |
22799 |
498/520（绿色） |
200tests |
|
22817 |
534/619（红色） |
100tests |
|
22820 |
Multiple Colors |
3*100tests |
Caspase binding assay kit
Product Name |
Cat. # |
Ex/Em（nm） |
Size |
Cell Meter™ Live Cell Caspase 1 Binding Assay Kit |
20108 |
492/514 |
25tests |
Cell Meter™ Live Cell Caspase 2 Binding Assay Kit |
20111 |
492/514 |
25tests |
Cell Meter™ Live Cell Caspase 3/7 and Phosphatidylserine Detection Kit |
22850 |
Multiple Colors |
100tests |
Cell Meter™ Live Cell Caspase 3/7 Binding Assay Kit Green Fluorescence |
20100 |
492/514 |
25tests |
Cell Meter™ Live Cell Caspase 3/7 Binding Assay Kit Red Fluorescence |
20101 |
556/574 |
25tests |
Cell Meter™ Live Cell Caspase 6 Binding Assay Kit |
20113 |
492/514 |
25tests |
Cell Meter™ Live Cell Caspase 8 Binding Assay Kit |
20115 |
492/514 |
25tests |
Cell Meter™ Live Cell Caspase 9 Binding Assay Kit |
20117 |
492/514 |
25tests |
Cell Meter™ Live Cell Caspase 10 Binding Assay Kit |
20119 |
492/514 |
25tests |
Cell Meter™ Live Cell Caspase 13 Binding Assay Kit |
20125 |
492/514 |
25tests |
The detection of caspase binding activities with Kits 20101 and 22791. The fluorescence image analysis indicated the increased expression of caspase 3/7 (red, stained by TF3-DEVD-FMK) and Annexin V (green, stained by Annexin V-iFluor™ 488) in Jurkat cells induced by 1μM staurosporine for 3 hours. The fluorescence images of the cells (300,000 cells/well) were taken with Olympus fluorescence microscope using the DAPI, FITC, and TRITC channel respectively. Individual images of the same cell population were merged as shown above. A: Non-induced control cells; B: Double staining of staurosporine-induced cells for caspase 3/7 (red) and Annexin V (green); C. Triple staining of staurosporine-induced cells for caspase 3/7 (red), Annexin V (green) and nucleus (blue).
▍▏Caspase 底物和抑制剂
Product Name |
Cat. # |
Biological Function |
Size |
Ex/Em (nm) |
Ac-DEVD-AFC |
13401 |
Fluorogenic Caspase 3/7 Substrate |
5 mg |
380/500 |
Ac-DEVD-AMC |
13402 |
Fluorogenic Caspase 3/7 Substrate |
5 mg |
351/430 |
Ac-DEVD-CHO |
13403 |
Caspase 3/7 Inhibitor |
1 mg |
N/A |
Ac-DEVD-pNA |
13405 |
Chromogenic Caspase 3/7 Substrate |
5 mg |
408 |
Ac-IETD-AFC |
13410 |
Fluorogenic Caspase 8 Substrate |
5 mg |
380/500 |
Ac-IETD-AMC |
13411 |
Fluorogenic Caspase 8 Substrate |
5 mg |
351/430 |
Ac-IETD-CHO |
13412 |
Caspase 8 Inhibitor |
5 mg |
N/A |
(Ac-IETD)2-R110 |
13431 |
Fluorogenic Caspase 8 Substrate |
1 mg |
498/520 |
Ac-LEHD-AMC |
13426 |
Fluorogenic Caspase 9 Substrate |
5 mg |
351/430 |
(Ac-LEHD)2-R110 |
13427 |
Fluorogenic Caspase 9 Substrate |
1 mg |
498/520 |
Z-DEVD-AFC |
13420 |
Fluorogenic Caspase 3/7 Substrate |
5 mg |
380/500 |
Z-DEVD-AMC |
13421 |
Fluorogenic Caspase 3/7 Substrate |
5 mg |
351/430 |
Z-DEVD-pNA |
13422 |
Chromogenic Caspase 3/7 Substrate |
5 mg |
408 |
Z-IETD-AFC |
13425 |
Fluorogenic Caspase 8 Substrate |
5 mg |
380/500 |
Z-IETD-pNA |
13413 |
Chromogenic Caspase 8 Substrate |
5 mg |
408 |
Z-DEVD-ProRed™ 620 |
13433 |
Fluorogenic Caspase 3/7 Substrate |
1 mg |
534/619 |
(Z-DEVD)2-R110 |
13430 |
Fluorogenic Caspase 3/7 Substrate |
1 mg |
498/520 |
Z-IETD-ProRed™ 620 |
13434 |
Fluorogenic Caspase 8 Substrate |
1 mg |
534/619 |
Z-LEHD-ProRed™ 620 |
13435 |
Fluorogenic Caspase 9 Substrate |
1 mg |
534/619 |
The fluorescence imaging demonstrated the increase in FITC-C6-DEVD-FMK (Cat# 13408) fluorescence intensity with the addition of 1 μM staurospo- rine in Jurkat cells. Cells were incubated with FITC-C6-DEVD-FMK for 1 hour at 37°C.The fluorescence intensity of the cells (200,000 cells/100μL/well) was viewed under a fluorescence microscope using the FITC channel. A: Control; B:Staurosporine-treated.
▍▏还原型谷胱甘肽（GSH）检测
还原型谷胱甘肽在维持细胞的还原状态和正常生命活动中发挥着重要作用，如清除自由基，去除药物毒性，信号通路和增殖。GSH 浓度的降低是凋亡的早期信号。
Cell Meter™ Intracellular GSH Assay Kit 利用 Thiolite™ Green 与包内羟基反应便会发出强烈荧光的特性来检测 GSH 的浓度。随着胞浆内 Thiolite™ Green 的密度增加，会转移到凋亡细胞的线粒体内结合羟基发射荧光。该试剂盒可与其他检测细胞活性和凋亡的试剂同时使用，如 7 - ADD。适用于使用流式仪筛选凋亡抑制剂或激活剂。
Product Name |
Cat. # |
Ex/Em（nm） |
Size |
Amplite™ Fluorimetric Thiol Quantitation Assay Kit Green Fluorescence |
5524 |
490/515 |
1kit |
Cell Meter™ Intracellular GSH Assay KitOptimized for Flow Cytometry |
22810 |
490/515 |
25tests |
▍▏磷脂酰丝氨酸（phosphatidylserine，PS）检测
细胞凋亡会出现很多形态上的变化，细胞膜的变化是细胞凋亡早期信号之一。磷脂酰丝氨酸（PS) 本来表达在细胞膜的包内侧，随着凋亡的进行，逐渐转移到胞外。Annexins 是一种 Ca2+ 依赖的磷脂结合蛋白，其中 Annexin V 更加倾向于识别 PS。
Product Name |
Cat. # |
Ex/Em（nm） |
Size |
Cell Meter™ Annexin V Binding Apoptosis Assay Kit |
22828 |
405/450（蓝色） |
100tests |
Cell Meter™ Annexin V Binding Apoptosis Assay Kit |
22827（流式） |
650/668（深红） |
100tests |
Cell Meter™ Annexin V Binding Apoptosis Assay Kit |
22829 |
414/508（绿色） |
100tests |
Cell Meter™ Annexin V Binding Apoptosis Assay Kit |
22824（流式） |
490/520（绿色） |
100tests |
Cell Meter™ Annexin V Binding Apoptosis Assay Kit |
22830 |
424/560（橘色） |
100tests |
Cell Meter™ Annexin V Binding Apoptosis Assay Kit |
22825（流式） |
554/578（橘色） |
100tests |
Cell Meter™ Annexin V Binding Apoptosis Assay Kit |
22826（流式） |
590/610（红色） |
100tests |
Images of Jurkat cells in a Costar black wall/clear bottom 96-well plate stained with the Cell Meter™ AnnexinV Binding Apoptosis Assay Kit Red Fluorescence (Cat# 22826). A: Untreated control cells B: Cells treated with 1 μM staurosporine for 5 hours.
▍▏线粒体膜电位 Mitochondrion Membrane Potential (MMP) Assay
Cell Meter™ 线粒体膜电位检测试剂盒利用阳离子线粒体荧光探针检测膜电位的变化。在活细胞中荧光探针会逐渐积累，红色荧光强度会逐渐增强，而在凋亡细胞内膜通透性转运孔打开，膜电位崩溃，荧光信号逐渐减弱。
Product Name |
Cat. # |
Ex/Em（nm） |
Size |
Cell Meter™ JC-10™ Mitochondrion Membrane Potential Assay Kit |
22801（流式） |
510/525 |
100tests |
22800（荧光酶标仪） |
510/525 |
500tests |
|
Cell Meter™ Mitochondrion Membrane Potential Assay Kit |
22804（流式） |
546/575（橘色） |
100tests |
22805（荧光酶标仪） |
546/575（橘色） |
500tests |
|
Cell Meter™ NIR Mitochondrion Membrane Potential Assay Kit |
22802（流式） |
646/659 |
100tests |
22803（荧光酶标仪） |
646/659 |
- |
The detection of FCCP-induced mitochondrion membrane potential changes with Cell Meter™ JC-10™ Mitochondrion Membrane Potential Assay Kit Optimized for Flow Cytometry Assays (Cat# 22801) in Jurkat cells. Jurkat cells were dye loaded with JC-10™ dye-loading solution along with DMSO (left) or 20 μM FCCP(right) for 10 minutes. The fluorescence intensities for both J-aggregates and monomeric form JC-10™ were measured with FACSCalibur™ FLOW cytometer (Becton Dickinson) using FL1 and FL2 channels after compensation.
▍▏TUNEL Apoptosis Assay
DNA 片段化是凋亡晚期的主要特征。TUNEL 检测原理是基因组 DNA 断裂时，暴露的 3'-OH 可以在末端脱氧核苷酸转移酶 (Terminal Deoxynucleotidyl Transferase, TdT) 的催化下加上荧光素 (FITC) 标记的 dUTP (fluorescein-dUTP)，从而可以通过荧光显微镜或流式细胞仪进行检测。目前市场上的 TUNEL 都含有具有毒性的二甲胂酸钠，可引起细胞凋亡或 DNA 合成的减少。而 AAT 的 Cell Meter™ TUNEL Apoptosis Assay Kit 使用特有的不含该毒性物质的缓冲系统。
Product Name |
Cat. # |
Ex/Em（nm） |
Size |
Cell Meter™ TUNEL Apoptosis Assay Kit |
22844 |
556/579 |
50tests |
The analysis of nuclear apoptosis with Cell Meter™ TUNEL Apoptosis Assay Kit(Cat# 22844). The fluorescence imaging demonstrated the increase in TUNEL reaction with the addition of 1μM staurosporine for 2 hours (B) or 4 hours (C) compared to control (A) in HeLa cells. Cells were incubated with reaction mixture for 1 hour at 37°C. The fluorescence intensity of the cells (30,000 cells/100 μL/well) was analyzed under a fluorescence microscope using the TRITC channel. DNA strand breaks were shown as more intense fluorescent staining spots in cells treated with staurosporine.
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