AAT Bioquest®：活性氧检测相关产品

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AAT Bioquest 22900 22901 22902 22903 22904 11306 11552 16060 22970 22971 16055 16350 16351 16356 11503 11506 11504 11505

活性氧检测

AAT 提供最全活性氧产品线，可检测总活性氧也可选择性检测。高敏感性高特异性，只需一步，一个小时即可得到结果。

▍▏总 ROS 检测试剂盒

Product Name	Cat. #	Ex/Em（nm）	Size
Cell Meter™Fluorimetric Intracellular Total ROS Activity Assay Kit	22900	492/520（绿色）	200tests
Cell Meter™Fluorimetric Intracellular Total ROS Activity Assay Kit	22901	520/605（红色）	200tests
Cell Meter™Fluorimetric Intracellular Total ROS Activity Assay Kit	22902	556/566（橘红）	200tests
Cell Meter™Fluorimetric Intracellular Total ROS Activity Assay Kit	22903	658/675（深红）	200tests
Cell Meter™Fluorimetric Intracellular Total ROS Activity Assay Kit	22904（流式）	490/520（绿色）	200tests

Images of HeLa cells stained with Cell Meter™ Fluorimetric Intracellular Total ROS Activity Assay Kit (Cat# 22903) in a Costar 96-well black wall/clear bottom plate. Left: Untreated control cells. Right: Cells treated with 100 μM tert-butyl hydroperoxide (TBHP) for 30 minutes before staining.

▍▏氧化酶检测

Product Name	Cat. #	Ex/Em（nm）	Size
Amplite™ Fluorimetric Catalase Assay Kit	11306	571/585	200tests
Amplite™ Fluorimetric Peroxidase Assay Kit Red Fluorescence	11552	571/585	200tests

▍▏可选择性检测各种活性氧

Product Name	Cat. #	Ex/Em（nm）	Size
线粒体过氧化物检测试剂盒Cell Meter™Fluorimetric Mitochondrial Superoxide Activity Assay Kit	16060	509/534（绿色）	200tests
	22970（流式）	540/590（橘红）	200tests
	22971（酶标仪）	540/590（橘红）	200tests
线粒体羟基自由基检测试剂盒 Cell Meter™ Mitochondrial Hydroxyl Radical Detection Kit Red Fluorescence	16055	576/598（红色）	200tests
NO 检测试剂盒 Cell Meter™Fluorimetric Intracellular Nitric Oxide (NO) Activity Assay Kit	16350（酶标仪）	540/590（橘红）	100tests
	16351（流式）	488/590（橘红）	100tests
	16356（流式）	630/660（红色）	100tests
过氧化氢检测试剂盒 Cell Meter™Intracellular Fluorimetric Hydrogen Peroxide Assay Kit	11503	492/515（绿色）	200tests
	11506	490/530（绿色）	100tests
	11504	405/450（蓝色）	100tests
	11505（流式）	405/450（蓝色）	100tests

Fluorescence images of hydroxyl radical measurements in HeLa cells using Cell Meter™ Mitochondrial Hydroxyl Radical Detection Kit (Cat# 16055).

Control (left): Hela cells were kept in 1X HBSS buffer without treatment. Cell nuclei were stained with Hoechst 33342 (Blue, Cat# 17530).

Fenton Reaction (right): Cells were treated with 10 μM CuCl₂ and 100 μM H₂O₂ in 1X HBSS buffer at 37°C for 1 hour.

Detection of exogenous nitric oxide (NO) in Jurkat cells upon DEA NONOate treatment (NO donor) using Cell Meter™ Fluorimetric Intracellular Nitric Oxide Activity Assay Kit (Cat#16360). Cells were incubated with Nitrixyte™ NIR at 37 °C, 5% CO₂ for 30 minutes. Spin down and wash cells with Hanks and 10 mM HEPES buffer. Cells were further treated with or without 1 mM DEA NONOate in Assay Buffer (Component C) at 37 °C, 5% CO₂ incubator for 60 minutes.