【Yeastern】EZtime Real-Time PCR Premix

FYT103-100P (100 preps)

EZtime Real-Time PCR 1.25 ml
Premix (2×, SYBR Green)

FYT103-400P (400 preps)

EZtime Real-Time PCR 1.25 ml x 4
Premix (2×, SYBR Green)

FYT104-100P (100 preps)

EZtime Real-Time PCR 1.25 ml
Premix (2×, SYBR Green, ROX)

FYT104-400P (400 preps)

EZtime Real-Time PCR 1.25 ml x 4
Premix (2×, SYBR Green, ROX)

FYT105-100P (100 preps)

EZtime Real-Time PCR 1.25 ml
Premix (2×, TaqMan)

FYT105-400P (400 preps)

EZtime Real-Time PCR 1.25 ml x 4
Premix (2×, TaqMan)

FYT106-100P (100 preps)

EZtime Real-Time PCR 1.25 ml
Premix (2×, TaqMan, ROX)

FYT106-400P (400 preps)

EZtime Real-Time PCR 1.25 ml x 4
Premix (2×, TaqMan, ROX)

Premix contains:
• Hotstart Taq DNA polymerase
• SYBR Green real-time PCR Buffer
• dNTP mix including dATP, dCTP, dGTP, dTTP
• 5 mM MgCl₂

The EZtime Real-Time PCR Premix (# FYT103-100P or # FYT104-100P) is a ready-to-use, 2× concentrated PCR premix, including Hotstart Taq, SYBR Green I, optimized reaction buffer and nucleotides (# FYT104-100P with ROX) for running quantitative real-time PCR, including qPCR and 2-step qRT-PCR, in the SYBR Green I detection format. SYBR Green Premix provides high sensitivity, wide dynamic range and reproducibility for quantification.

The EZtime Real-Time PCR Premix (# FYT105-100P or # FYT106-100P) is a ready-to-use, 2× concentrated PCR premix that contains all reagents (except for primers, probe and template) needed for running real-time PCR (# FYT106-100P with ROX). Real-time PCR is performed by addition of various probes, such as TaqMan, Molecular Beacon, etc. This product combines the high performance Hotstart Taq with a buffer which provides good specificity, amplification efficiency for real-time PCR.

• Specificity : Hotstart Taq and the optimized buffer eliminates non-specific amplification and formation of primer dimers

• Sensitivity : detects low copy number targets

• Wide linear range : accurate quantification across 9 orders of magnitude

• Reproducibility and convenience : ready-to-use 2× master mix minimizes pipetting error and reduces set-up time

• Quantitative real-time PCR

• Quantitative 2-step RT-PCR

• Quick and accurate detection and quantification of target gene through realtime PCR

• Error<0.01, 2.1>Efficiency>1.90, ∆CP<1

• The absence of endo-, exodeoxyribonucleases and ribonucleases is confirmed by appropriate quality tests. Functionally tested in qPCR for specificity, sensitivity and reproducibility using serial dilutions of control genomic DNA template.

Primers were designed to amplify a specific region of YB T&A™ Vector that has been serially diluted to different concentrations.