【Minerva Biolabs】ZellShield®用于预防细菌和真菌污染的细胞培养补充剂

浏览量：161

Minerva Biolabs ZellShield® Cat. No. 13-0050 Cat. No. 13-0150

ZellShield^®

用于预防细菌和真菌污染的细胞培养补充剂

ZellShield^®是一种用于培养基的即用添加剂，用于保护细胞培养免受细菌、真菌、酵母和支原体等广泛常见污染物的影响。这种保护效果是通过一种具有抗生素和抗真菌性质以及可忽略副作用的基于一种具有大环内酯特性的新型抗生素的组合，该抗生素抑制细菌的DNA和蛋白质合成。当ZellShield®按照如下操作添加到培养基中时，不需要向培养基添加传统抗生素，如青霉素、链霉素、制霉菌素、两性霉素B等。ZellShield^®可以作为添加剂直接添加到培养基中以保护细胞培养。

ZellShield^®产品信息
包装规格	50 ml（Cat. No. 13-0050）——足以配制5L培养基
包装规格	3 x 50 ml（Cat. No. 13-0150）——足以配制15L培养基
推荐用途	仅适用于细胞培养的研究和工业领域。不建议用于临床应用。
贮存	ZellShield^® 必须在≤-18°C条件下储存（用冷藏袋运输）且对光敏感，必须避光。低于-18°C条件下ZellShield^®可长期储存，如加入至培养基后，在4-8°C条件下最多储存4周。
优点	●特异性：ZellShield^®对大多数细胞内和细胞外生长的革兰氏阴性和革兰氏阳性细菌、支原体、原虫、真菌和酵母菌具有活性
	●广泛的应用范围：ZellShield^®可用于永久细胞培养以及防止新鲜制备的初级细胞培养受到污染
	●无细胞毒性：到目前为止，没有报告在各种细胞系上产生细胞毒性效应，前提是按照说明书使用该产品
	●易于使用：将ZellShield^®直接添加到新鲜制备的细胞培养基（例如烧瓶）中，或者与细胞培养基预混
	●低抗性风险：ZellShield^®含有精心选择的抗微生物剂，其抗性偏倧极低。它们的联合活性降低了抗性突变体存活的概率
	●稳定性：该试剂在典型的孵育时间和条件下稳定和活性

使用步骤：

1 .ZellShield^®无菌溶液是 100 倍浓缩液，可添加到细胞培养基中。一瓶 50毫升的产品足够用于 5 升的培养基。

2 .ZellShield^®可以直接添加到任何动物细胞培养的烧瓶或培养皿中，最好在更换培养基或细胞传代后添加。在 37°C 孵育 7 天后，应更换含 ZellShield^®新鲜培养基以达到最大的保护效果。

3 .ZellShield^®含有一种氟喹诺酮，可与其他用作耐药标记的抗生素一起使用。其他抗生素成分不用作耐药标记。可以额外使用青霉素/链霉素（不建议）。

英文说明书

ZellShield®

Cell culture supplement for prevention of bacterial and fungal contaminations

1、Background

ZellShield® is a ready-to-use multi-functional supplement, for protection of cell cultures from a broad range of common contaminants such as bacteria, fungi, yeast, and mycoplasma. This protective effect is achieved thanks to a combination of novel components with antibiotic and antimycotic properties and negligible side effects. When ZellShield® is added to the culture medium as described below, the application of conventional antibiotics such as penicillin, streptomycin, nystatin etc. can safely be omitted.

ZellShield® can be used routinely as additive directly to the medium to safeguard cell cultures.

2、Precautions and Storage

ZellShield® is for research use only. Avoid contact to eyes and skin. Rinse with water in case of direct contact.Wear gloves while handling this product. Do not swallow.

ZellShield® is shipped on cool packs and must be stored ≤-18 °C. Cell culture medium supplemented with ZellShield® can be stored at +2 °C to +8 °C for up to 4 weeks. This product is light sensitive and should be stored accordingly.

3、Instructions for Use

The application of ZellShield® is very simple: the sterile solution is a 100-fold concentrate to be added to the cell culture medium. One bottle with 50 ml is sufficient for 5 liters of culture medium.

ZellShield® can be added directly to any flask or plate of animal cell cultures, preferably when changing the medium or after passaging cells. ZellShield® should be refreshed after 7 days of incubation at 37 °C to achieve maximum protection.

ZellShield® contains a fluoroquinolone and can be used in conjunction with other antibiotics used as resistance marker. The other antibiotic components are not used as resistance markers. An additionalapplication of Pen/Strep is feasible but not recommended.

Cat. No.:

13-0050 (50 ml – sufficient for 5 liters of culture medium)

13-0150 (3 × 50 ml – sufficient for 15 liters of culture medium)

4、PROCEDURE – OVERVIEW